Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.
PLoS One. 2012;7(3):e33561. doi: 10.1371/journal.pone.0033561. Epub 2012 Mar 16.
Viral polymerase entry and pausing at intergenic junctions is predicted to lead to a defined polarity in the levels of rhabdovirus gene expression. Interestingly, we observed that the rabies virus glycoprotein mRNA is differentially over-expressed based on this model relative to other transcripts during infection of 293T cells. During infection, the rabies virus glycoprotein mRNA also selectively interacts with the cellular poly(rC)-binding protein 2 (PCBP2), a factor known to influence mRNA stability. Reporter assays performed both in electroporated cells and in a cell-free RNA decay system indicate that the conserved portion of the 3' UTR of the rabies virus glycoprotein mRNA contains an RNA stability element. PCBP2 specifically interacts with reporter transcripts containing this 72 base 3' UTR sequence. Furthermore, the PCBP2 interaction is directly associated with the stability of reporter transcripts. Therefore, we conclude that PCBP2 specifically and selectively interacts with the rabies virus glycoprotein mRNA and that this interaction may contribute to the post-transcriptional regulation of glycoprotein expression.
病毒聚合酶进入和在基因间连接处暂停，预计会导致弹状病毒基因表达水平呈现出明确的极性。有趣的是，我们观察到，相对于感染 293T 细胞时的其他转录本，狂犬病病毒糖蛋白 mRNA 根据该模型呈现出差异过表达。在感染过程中，狂犬病病毒糖蛋白 mRNA 还选择性地与细胞多聚（rC）结合蛋白 2（PCBP2）相互作用，PCBP2 是一种已知影响 mRNA 稳定性的因子。在电穿孔细胞和无细胞 RNA 降解系统中进行的报告基因检测表明，狂犬病病毒糖蛋白 mRNA 的 3'UTR 保守部分含有一个 RNA 稳定性元件。PCBP2 特异性地与包含该 72 个碱基 3'UTR 序列的报告基因转录本相互作用。此外，PCBP2 的相互作用与报告基因转录本的稳定性直接相关。因此，我们得出结论，PCBP2 特异性和选择性地与狂犬病病毒糖蛋白 mRNA 相互作用，这种相互作用可能有助于糖蛋白表达的转录后调控。
