Department of Chemistry, Capital Normal University, Beijing 100048, China.
Department of Chemistry, Capital Normal University, Beijing 100048, China.
Biosens Bioelectron. 2012 May 15;35(1):470-474. doi: 10.1016/j.bios.2012.02.061. Epub 2012 Mar 7.
The long-term stability of sensing interfaces is an important issue in biosensor fabrication. A novel stable gold nanoparticle (AuNP)-modified glassy carbon (GC) electrode interface (GC-Ph-AuNP)-based biosensor for detecting carcinoembryonic antigen (CEA) was developed. GC electrodes were modified with 1,4-phenylenediamine to form a stable layer, and then AuNPs were bound onto the GC electrodes through CAu bonds. Anti-CEA was directly adsorbed on AuNPs fixed on the GC electrode. The linear range of the immunosensor was from 10 fg to 100 ng mL(-1) with a detection limit of 3 fg mL(-1) (S/N=3). The current of the immunosensor was increased by 4% after one month. The GC-Ph-AuNP immunosensor showed high sensitivity, a wide linear range, low detection limit, and good selectivity and stability. The immobilization method of the immunosensor could be widely applied to construct other immunosensors.
传感界面的长期稳定性是生物传感器制造中的一个重要问题。本研究开发了一种新型稳定的基于金纳米粒子（AuNP）修饰玻碳（GC）电极界面（GC-Ph-AuNP）的用于检测癌胚抗原（CEA）的生物传感器。GC 电极通过 1,4-苯二胺进行修饰以形成稳定层，然后通过 CAu 键将 AuNPs 结合到 GC 电极上。抗 CEA 直接吸附在固定在 GC 电极上的 AuNPs 上。免疫传感器的线性范围为 10 fg 至 100 ng mL(-1)，检测限为 3 fg mL(-1)（S/N=3）。一个月后，免疫传感器的电流增加了 4%。GC-Ph-AuNP 免疫传感器具有高灵敏度、宽线性范围、低检测限以及良好的选择性和稳定性。该免疫传感器的固定化方法可以广泛应用于构建其他免疫传感器。
