Row K L, Johnson R B
Department of Preventive Dental Science, University of Manitoba, Winnipeg, Canada.
Am J Anat. 1990 Oct;189(2):179-88. doi: 10.1002/aja.1001890208.
Maxillary right first molar teeth of rats were tipped mesially with an orthodontic appliance for 2 weeks (experimental group), 3H-proline was injected, and orthodontic forces were removed 6 hr later (time 0). The contralateral molar teeth of treated (internal control group) and age- and weight-matched untreated animals (external control group) were also studied. Diastemata were created between the molar teeth by the orthodontic appliance, and transseptal fibers between first and second (P less than 0.001) and second and third molars (P less than 0.005) were significantly lengthened as compared to external and internal controls at time 0. Diastemata between molar teeth were closed 5 days after removal of orthodontic force. Transseptal fibers adjacent to the source of the orthodontic force (mesial region) had the highest mean number of 3H-proline-labeled proteins at time 0 and at all times following removal of the force (P less than 0.001), and had the highest rate of labeled protein removal (P less than 0.001). Half-lives for removal of 3H-proline-labeled transseptal fiber proteins were significantly greater in mesial and distal regions and significantly less in middle regions of experimentals than in corresponding regions of external controls (P less than 0.001). These data suggest the following: 1) transseptal fibers adjust their length by rapid remodeling in regions experiencing a tensile force; 2) collagenous protein turnover within the middle third of the transseptal fibers is more rapid subsequent to release of orthodontic force than during normal physiologic drift, suggesting that this region adapts rapidly to changes in adjacent tooth position and that these fibers do not play a significant role in relapse of orthodontically relocated teeth; and 3) significant differences in turnover rates of 3H-proline-labeled transseptal ligament proteins of external and internal control quadrants suggest that tooth movement produces both local and systemic effects on collagenous protein metabolism.
用正畸矫治器将大鼠右上颌第一磨牙向近中倾斜2周（实验组），注射3H-脯氨酸，6小时后去除正畸力（时间0）。还研究了处理组（内部对照组）以及年龄和体重匹配的未处理动物的对侧磨牙（外部对照组）。正畸矫治器在磨牙之间形成间隙，与外部和内部对照组相比，第一和第二磨牙之间（P<0.001）以及第二和第三磨牙之间的跨龈纤维（P<0.005）在时间0时显著延长。去除正畸力5天后磨牙之间的间隙关闭。在时间0以及去除力后的所有时间，靠近正畸力源（近中区域）的跨龈纤维中3H-脯氨酸标记蛋白的平均数量最高（P<0.001），并且标记蛋白去除率最高（P<0.001）。实验组近中及远中区域3H-脯氨酸标记的跨龈纤维蛋白去除的半衰期显著长于外部对照组的相应区域，而中间区域则显著短于外部对照组的相应区域（P<0.001）。这些数据表明：1）跨龈纤维通过在承受拉力的区域快速重塑来调节其长度；2）正畸力释放后，跨龈纤维中间三分之一内的胶原蛋白质周转比正常生理漂移时更快，这表明该区域能迅速适应相邻牙齿位置的变化，并且这些纤维在正畸移动牙齿的复发中不起重要作用；3）外部和内部对照象限中3H-脯氨酸标记的跨龈韧带蛋白周转率的显著差异表明，牙齿移动对胶原蛋白质代谢产生局部和全身影响。
