Johnson R B
Department of Anatomy, Faculty of Dentistry, University of Manitoba, Winnipeg, Canada.
Anat Rec. 1989 Feb;223(2):121-7. doi: 10.1002/ar.1092230203.
The distribution of 35S-sulfate-labeled macromolecules was examined within three regions of the transseptal ligament: the 1) mesial, 2) middle and 3) distal thirds. Swiss mice, 6 weeks of age, were injected with 35S-sulfate and killed after 1, 6, and 12 hours and 1, 2, 3, 4, 5, and 7 days. Silver grains and cell nuclei were counted on autoradiographs which had been counterstained by the Van Gieson method, and mean counts were analyzed statistically. Analysis of variance revealed no significant differences in mean number of cell nuclei between regions throughout the course of the experiment. 35S-sulfate was rapidly incorporated into the transseptal ligament macromolecules. Grain counts were highest 6 hours after injections: counts were highest over the middle and lowest over the mesial thirds of the ligament. The rate of grain removal was significantly higher in the middle third compared to the mesial or distal thirds (P less than 0.001) and was significantly lower in the mesial third compared to the middle or distal thirds (P less than 0.001). The half-life of labeled macromolecules was significantly greater in the mesial and distal thirds than in the middle third (P less than 0.005). The data demonstrate significantly higher rates of turnover of 35S-sulfate-labeled macromolecules in the middle region of the transseptal ligament. Since cellular density was similar throughout the transseptal ligament, higher turnover rates of 35S-sulfate-labeled macromolecules probably indicate higher rates of cellular activity in this region, possibly a result of tissue remodeling coincident to stresses generated by occlusal forces and physiologic drift of the adjacent teeth.
研究了经隔韧带三个区域内35S - 硫酸盐标记大分子的分布情况：1）近中三分之一、2）中间三分之一和3）远中三分之一。选用6周龄的瑞士小鼠，注射35S - 硫酸盐，分别在1、6和12小时以及1、2、3、4、5和7天后处死。在经Van Gieson法复染的放射自显影片上计数银颗粒和细胞核，并对平均计数进行统计学分析。方差分析显示，在整个实验过程中，各区域之间细胞核的平均数量没有显著差异。35S - 硫酸盐迅速掺入经隔韧带大分子中。注射后6小时颗粒计数最高：韧带中间三分之一处计数最高，近中三分之一处最低。与近中或远中三分之一相比，中间三分之一处的颗粒清除率显著更高（P小于0.001），而与中间或远中三分之一相比，近中三分之一处的颗粒清除率显著更低（P小于0.001）。标记大分子的半衰期在近中和远中三分之一处显著长于中间三分之一处（P小于0.005）。数据表明，经隔韧带中间区域35S - 硫酸盐标记大分子的周转速率显著更高。由于整个经隔韧带的细胞密度相似，35S - 硫酸盐标记大分子的较高周转速率可能表明该区域细胞活性较高，这可能是由咬合力量和相邻牙齿的生理漂移产生的应力导致组织重塑的结果。
