Johnson R B
Department of Anatomy, University of Manitoba, Faculty of Dentistry, Winnipeg, Canada.
Anat Rec. 1989 Oct;225(2):87-95. doi: 10.1002/ar.1092250202.
There is little information concerning the effect of altered occlusal forces on the turnover of collagenous proteins of transseptal fibers of the periodontium. In the present study, hypofunction was induced in rats by extraction of the maxillary teeth, allowing the mandibular teeth to supererupt (hypofunctional side, herein). The contralateral side served as an internal control, although it was likely experiencing occlusal hyperfunction (hyperfunctional side, herein). Untreated animals were also studied (external controls, herein). Animals were injected with 3H-proline and silver grains were counted on radioautographic preparations. The study demonstrated significant differences in the synthesis and degradation of collagenous proteins coincident to altered occlusal function; 3H-proline was most heavily incorporated into the transseptal fibers of hyperfunctional and least rapidly into the external control tissues (P less than .001). Significant differences in grain counts were evident during the first 3 weeks after injections. Collagenous proteins were degraded most rapidly in transseptal fibers of the hyperfunctional and least rapidly in hypofunctional tissues (P less than .001). The study also demonstrated regional variability in the turnover of labeled collagenous proteins, that is, proteins were synthesized and degraded most rapidly in the middle third and least rapidly in the mesial third of the ligament (P less than .001). "Whole" counts (mean of counts over middle, mesial, and distal thirds) were not similar to those of any specific region and could provide erroneous information concerning remodeling of collagenous proteins of transseptal fibers. Transseptal fibers, labeled by the 3H-proline pulse, migrated occlusally with the teeth; new transseptal fibers and bone were formed at the crest of the interdental septum.
关于改变的咬合力对牙周膜中跨牙槽纤维胶原蛋白质更新的影响，相关信息较少。在本研究中，通过拔除上颌牙齿诱导大鼠功能减退，使下颌牙齿过度萌出（此处称为功能减退侧）。对侧作为内部对照，尽管其可能正经历咬合功能亢进（此处称为功能亢进侧）。还对未处理的动物进行了研究（此处称为外部对照）。给动物注射³H - 脯氨酸，并在放射自显影片上计数银颗粒。该研究表明，胶原蛋白质的合成和降解与咬合功能改变相关存在显著差异；³H - 脯氨酸在功能亢进侧的跨牙槽纤维中掺入量最多，而在外部对照组织中掺入速度最慢（P＜0.001）。注射后前3周银颗粒计数存在明显差异。胶原蛋白质在功能亢进侧的跨牙槽纤维中降解最快，而在功能减退组织中降解最慢（P＜0.001）。该研究还表明标记的胶原蛋白质更新存在区域差异，即蛋白质在韧带中间三分之一处合成和降解最快，而在近中三分之一处最慢（P＜0.001）。“整体”计数（中间、近中和远中三分之一处计数的平均值）与任何特定区域的计数都不相似，可能会提供有关跨牙槽纤维胶原蛋白质重塑的错误信息。经³H - 脯氨酸脉冲标记的跨牙槽纤维随牙齿向咬合面迁移；在牙间间隔嵴处形成了新的跨牙槽纤维和骨。
