CSIR, Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India.
J Ethnopharmacol. 2012 May 7;141(1):542-6. doi: 10.1016/j.jep.2012.02.040. Epub 2012 Mar 15.
The anti-inflammatory activity of Aloe vera was investigated through MMP inhibition studies. The effect of Aloe vera on MMP-9 inhibition was tested on peripheral blood mononuclear cells (PBMC).
Peripheral blood mononuclear cells (PBMC) were isolated from the heparinised venous blood by Ficoll Diatrizoate gradient centrifugation. The cell count and viability was determined using dye exclusion technique. Cytotoxicity was evaluated by MTT assay. Activation of MMP-9 was visualized by gelatin zymography. Inhibition of MMP-9 in the presence of aqueous extract of Aloe vera was detected by gelatin zymography and confirmed by RT-PCR.
Peripheral blood mononuclear cells (PBMC) showed significant inhibition in the activity of MMP-9, indicating the in vitro inhibitory effect of Aloe vera on MMP-9. Zymographic analysis and RT-PCR showed that it caused a significant reduction in the production of MMP-9 in a concentration dependent manner.
The inhibition of MMP-9 production in the cells was detected by gelatin zymography and was confirmed by RT-PCR.
通过 MMP 抑制研究来研究芦荟的抗炎活性。通过对外周血单核细胞 (PBMC) 的 MMP-9 抑制测试来测试芦荟对 MMP-9 的抑制作用。
通过 Ficoll Diatrizoate 梯度离心从肝素化静脉血中分离外周血单核细胞 (PBMC)。使用染料排除技术测定细胞计数和活力。通过 MTT 测定评估细胞毒性。通过明胶酶谱法可视化 MMP-9 的激活。通过明胶酶谱法和 RT-PCR 检测到在存在芦荟水提取物的情况下 MMP-9 的抑制。
外周血单核细胞 (PBMC) 显示 MMP-9 活性显著抑制，表明芦荟对 MMP-9 具有体外抑制作用。酶谱分析和 RT-PCR 表明，它以浓度依赖的方式显著降低 MMP-9 的产生。
通过明胶酶谱法检测到细胞中 MMP-9 产生的抑制，并通过 RT-PCR 得到证实。
