The effects of oral on the efficacy of transplanted human endothelial cells and the expression of matrix metalloproteinases in diabetic wound healing.

BACKGROUND

Diabetic wounds are characterized by delayed healing and impaired angiogenesis. and human umbilical vein endothelial cells (HUVECs) are reported to facilitate wound healing, and the former also has hypoglycemic property. Matrix metalloproteinases are enzymes that play a role in diabetic wound pathogenesis.

OBJECTIVE

To investigate whether oral can enhance the efficacy of HUVEC transplantation and inhibit the expression of matrix metalloproteinases in wound healing of diabetic mice.

MATERIALS AND METHODS

BALB/c nude mice were randomly assigned into five groups: normal control group, diabetic group (DM), DM transplanted with HUVECs, DM treated with oral , and DM treated with combined HUVECs and oral . Diabetes was induced by streptozotocin. Bilateral full-thickness excision cutaneous wounds were created. At days 7 and 14 post-wounding, the following parameters were determined: blood glucose, wound area, wound perfusion, capillary vascularity, re-epithelialization rate and tissue VEGF levels. Tissue expressions of MMP-2 and MMP-9 were compared between the DM mice and those treated with oral .

RESULTS

Over days 7 and 14, exerted glucose-lowering effect in diabetic mice. Higher wound closure rate, blood flow and capillary vascularity, and lower MMP-2 and MMP-9 expressions were observed at both time points in DM treated with group compared with DM group ( < 0.05). Moreover, combined therapy of HUVECs and oral was more effective than or HUVECs alone in increasing VEGF levels, capillary vascularity and wound perfusion. Blood glucose levels were negatively correlated with angiogenesis ( = 0.000.

CONCLUSION

It is suggested that oral enhances the efficacy of HUVEC transplantation on diabetic wound angiogenesis, partly through improving glycemic control. Oral also promotes diabetic wound healing via inhibition of MMP-2 and MMP-9 expressions.