Gay Olivia, Nakamura Fumihiko, Baudier Jacques
Commun Integr Biol. 2011 Nov 1;4(6):791-5. doi: 10.4161/cib.17911.
The Refilins (RefilinA and RefilinB) are a novel family of short-lived actin regulatory proteins that are expressed during changes in cellular phenotype such as epithelial to mesenchymal transition (EMT). The Refilins promote to the formation of actin- and myosin-rich perinuclear bundles that are characteristic of cellular phenotypic switches. In epithelial cells, RefilinB is up-regulated in response to TGF-β stimulation and function in organization of apical perinuclear actin fibers during early stage of the EMT process1. In fibroblasts, RefilinB stabilizes perinuclear parallel actin bundles which resemble actin cap 2. Refilins bind and modulate the function of Filamin A (FLNA). Upon binding to Refilins, FLNA is capable of assembling actin filaments into parallel bundles, possibly by undergoing conformational changes at the C-terminal. Perinuclear actin structures determine nuclear shape, cell morphology, cell adhesion and possibly cell proliferation and gene regulation. Identifying the role of Refilins in organizing perinuclear actin networks provides additional insight in the process of intracellular mechanotransduction that regulate changes in cellular phenotype such as those observed during EMT.
Refilins（RefilinA和RefilinB）是一类新型的短寿命肌动蛋白调节蛋白家族，在细胞表型变化（如上皮-间质转化，EMT）过程中表达。Refilins促进富含肌动蛋白和肌球蛋白的核周束的形成，这是细胞表型转换的特征。在上皮细胞中，RefilinB在TGF-β刺激下上调，并在EMT过程早期参与顶端核周肌动蛋白纤维的组织1。在成纤维细胞中，RefilinB稳定类似于肌动蛋白帽的核周平行肌动蛋白束2。Refilins结合并调节细丝蛋白A（FLNA）的功能。与Refilins结合后，FLNA能够将肌动蛋白丝组装成平行束，可能是通过在C端发生构象变化。核周肌动蛋白结构决定核形状、细胞形态、细胞黏附，可能还决定细胞增殖和基因调控。确定Refilins在组织核周肌动蛋白网络中的作用，为调节细胞表型变化（如EMT过程中观察到的变化）的细胞内机械转导过程提供了更多见解。
