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哺乳动物脂肪酸合酶基因的分子克隆及启动子区域的鉴定。

Molecular cloning of the mammalian fatty acid synthase gene and identification of the promoter region.

作者信息

Amy C M, Williams-Ahlf B, Naggert J, Smith S

机构信息

Children's Hospital Oakland Research Institute, CA 94609.

出版信息

Biochem J. 1990 Nov 1;271(3):675-9. doi: 10.1042/bj2710675.

DOI:10.1042/bj2710675
PMID:2244872
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1149615/
Abstract

Rat genomic clones encompassing the entire fatty acid synthase gene have been isolated and characterized. The gene is present in a single copy of approx. 20 kb. Genomic DNA sequencing, direct RNA sequencing and S1 nuclease analysis showed that transcription is initiated primarily 1274 nucleotides upstream from the translation start site and that the 87-nucleotide-long 5'-untranslated mRNA sequence is the same in liver, lung and mammary gland. The 5'-flanking region and first intron contain several sequence elements which may be involved in the transcriptional regulation of this gene.

摘要

已分离并鉴定出包含整个脂肪酸合酶基因的大鼠基因组克隆。该基因以单拷贝形式存在，约为20 kb。基因组DNA测序、直接RNA测序和S1核酸酶分析表明，转录主要起始于翻译起始位点上游1274个核苷酸处，并且87个核苷酸长的5'-非翻译mRNA序列在肝脏、肺和乳腺中是相同的。5'-侧翼区域和第一个内含子包含几个可能参与该基因转录调控的序列元件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2199/1149615/8e62a5b0fd42/biochemj00172-0113-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2199/1149615/fc5397497422/biochemj00172-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2199/1149615/4406e562051b/biochemj00172-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2199/1149615/8e62a5b0fd42/biochemj00172-0113-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2199/1149615/fc5397497422/biochemj00172-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2199/1149615/4406e562051b/biochemj00172-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2199/1149615/8e62a5b0fd42/biochemj00172-0113-b.jpg

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