Early Stage Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, CA, 94080, USA,
Cytotechnology. 2012 Dec;64(6):667-78. doi: 10.1007/s10616-012-9446-1. Epub 2012 Mar 27.
In recent years, several automated scale-down bioreactor systems have been developed to increase efficiency in cell culture process development. ambr™ is an automated workstation that provides individual monitoring and control of culture dissolved oxygen and pH in single-use, stirred-tank bioreactors at a working volume of 10-15 mL. To evaluate the ambr™ system, we compared the performance of four recombinant Chinese hamster ovary cell lines in a fed-batch process in parallel ambr™, 2-L bench-top bioreactors, and shake flasks. Cultures in ambr™ matched 2-L bioreactors in controlling the environment (temperature, dissolved oxygen, and pH) and in culture performance (growth, viability, glucose, lactate, Na(+), osmolality, titer, and product quality). However, cultures in shake flasks did not show comparable performance to the ambr™ and 2-L bioreactors.
近年来，已经开发出了几种自动化缩小规模的生物反应器系统，以提高细胞培养过程开发的效率。ambrTM 是一种自动化工作站，可在 10-15 毫升的一次性搅拌罐生物反应器中对单个培养物的溶解氧和 pH 进行单独监测和控制。为了评估 ambrTM 系统，我们在平行的 ambrTM、2-L 台式生物反应器和摇瓶中比较了四种重组中国仓鼠卵巢细胞系在分批补料过程中的性能。ambrTM 中的培养物在控制环境（温度、溶解氧和 pH）和培养性能（生长、活力、葡萄糖、乳酸盐、Na(+)、渗透压、滴度和产品质量）方面与 2-L 生物反应器相匹配。然而，摇瓶中的培养物的性能与 ambrTM 和 2-L 生物反应器不具有可比性。
