Marani E, Adriolo P J, Boon M E, Kok L P
Department of Physiology, University of Leiden, The Netherlands.
Eur J Morphol. 1990;28(2-4):121-31.
Microwave-stimulated enzyme incubations for acetylcholinesterase, 5'-nucleotidase, alkaline phosphatase, lactate dehydrogenase, malate dehydrogenase, succinic dehydrogenase and isocitric dehydrogenase were studied, and compared with incubations in a waterbath. Temperature settings of 37 degrees C and 50 degrees C were used, and the incubation times were varied from 30 seconds to 30 minutes. The desired temperature of the incubation solution was reached in the microwave oven within 1 minute, whilst in the waterbath it took 10 to 25 minutes. The microscopic results for alkaline phosphatase and succinic dehydrogenase at a temperature setting of 50 degrees C were superior in the microwave method for incubation times less than 15 minutes. It is postulated that the increased reaction product of alkaline phosphatase and succinic dehydrogenase is due to a temperature effect, which has to be large enough to be of practical value. For the other enzymes studied, microwave-stimulated incubations were no better than the conventional incubations at corresponding temperatures. For 5'-nucleotidase there were aspecific lead deposits in the microwave method. All enzymes performed at the elevated, unphysiological temperature of 50 degrees C proved to have advantages, except for 5'-nucleotidase, whilst for malate dehydrogenase there was an aspecific reduction of the colour developer at this temperature.
对微波刺激下乙酰胆碱酯酶、5'-核苷酸酶、碱性磷酸酶、乳酸脱氢酶、苹果酸脱氢酶、琥珀酸脱氢酶和异柠檬酸脱氢酶的孵育进行了研究，并与在水浴中孵育进行了比较。使用了37摄氏度和50摄氏度的温度设置，孵育时间从30秒到30分钟不等。在微波炉中1分钟内即可达到孵育溶液所需的温度，而在水浴中则需要10到25分钟。在50摄氏度温度设置下，对于孵育时间小于15分钟的情况，微波法中碱性磷酸酶和琥珀酸脱氢酶的显微镜检查结果更优。据推测，碱性磷酸酶和琥珀酸脱氢酶反应产物的增加是由于温度效应，该效应必须足够大才能具有实际价值。对于所研究的其他酶，微波刺激孵育并不比相应温度下的传统孵育更好。对于5'-核苷酸酶，微波法中有非特异性铅沉积。除5'-核苷酸酶外，所有在50摄氏度这个升高的、非生理温度下进行的酶实验都显示出优势，而对于苹果酸脱氢酶，在此温度下显色剂有非特异性减少。
