Abdel-Hafeez Ekhlas H, Ahmad Azza K, Ali Basma A, Moslam Fadia A
Department of Parasitology, Faculty of Medicine, Minia University, Minia 61111, Egypt.
Korean J Parasitol. 2012 Mar;50(1):57-62. doi: 10.3347/kjp.2012.50.1.57. Epub 2012 Mar 6.
A total of 450 stool samples were collected from inpatient and outpatient clinics of Pediatric Department, Minia University Hospital, Minia District, Egypt. Two groups of patients were studied, including 200 immunosuppressed and 250 immunocompetent children. Stool samples were subjected to wet saline and iodine mounts. A concentration technique (formol-ether sedimentation method) was carried out for stool samples diagnosed negative by wet saline and iodine mounts. Samples were stained by 2 different methods; acid fast stain (modified Ziehl-Neelsen stain) and Giemsa stain. Total 188 cases (94%) were diagnosed positive for parasitic infections among immunosuppressed children, whereas 150 cases (60%) were positive in immunocompetent children (P<0.0001). The most common protozoan infection in immunosuppressed group was Cryptosporidium parvum (60.2%), followed by Blastocystis hominis (12.1%), Isospora belli (9.7%), and Cyclospora caytenensis (7.8%). On the other hand, Entamoeba histolytica (24.6%) and Giardia lamblia (17.6%) were more common than other protozoans in immunocompetent children.
共从埃及米尼亚区米尼亚大学医院儿科门诊和住院部收集了450份粪便样本。对两组患者进行了研究，包括200名免疫抑制儿童和250名免疫功能正常的儿童。粪便样本进行了生理盐水湿片和碘液涂片检查。对生理盐水湿片和碘液涂片检查诊断为阴性的粪便样本采用浓缩技术（甲醛乙醚沉淀法）。样本采用两种不同方法染色；抗酸染色（改良齐-尼氏染色）和吉姆萨染色。免疫抑制儿童中共有188例（94%）被诊断为寄生虫感染阳性，而免疫功能正常的儿童中有150例（60%）为阳性（P<0.0001）。免疫抑制组最常见的原生动物感染是微小隐孢子虫（60.2%），其次是人芽囊原虫（12.1%）、贝氏等孢球虫（9.7%）和卡耶塔环孢子球虫（7.8%）。另一方面，在免疫功能正常的儿童中，溶组织内阿米巴（24.6%）和蓝氏贾第鞭毛虫（17.6%）比其他原生动物更常见。
