Varaschin Mary S, Hirsch Christian, Wouters Flademir, Nakagaki Karen Y, Guimarães Antônio M, Santos Domingos S, Bezerra Pedro S, Costa Rafael C, Peconick Ana P, Langohr Ingeborg M
Departament of Veterinary Medicine, Universidade Federal de Lavras, Lavras, MG, Brazil.
Korean J Parasitol. 2012 Mar;50(1):63-7. doi: 10.3347/kjp.2012.50.1.63. Epub 2012 Mar 6.
Congenital Neospora caninum infection was diagnosed in two Saanen goat kids from two distinct herds with a history of abortion and weak newborn goat kids in the Southern region of the State of Minas Gerais, Brazil. The first kid was weak at birth, had difficulty to rise and was unable to nurse. Gross lesions of porencephaly and hydrocephalus ex vacuo were seen. Multifocal necrosis, gliosis and non-supurative encephalitis were observed in the brain. Several parasitic cysts with a thick wall that reacted strongly only with polyclonal antiserum to Neospora caninum were seen in the cerebral cortex, brain stem and cerebellum. The second kid was born from a Neospora caninum seropositive mother that aborted in the last pregnancy. It was born without clinical signs. The diagnosis of neosporosis was based on antibody titer of 1:800 to N. caninum by indirect fluorescence antibody test obtained from blood collected before the goat kid ingested the colostrum and Neospora caninum DNA was detected by polymerase chain reaction and sequenced from placenta. This is the first report of neosporosis in goats in the southeast region of Brazil.
在巴西米纳斯吉拉斯州南部地区，从两个不同畜群的两只萨能山羊幼崽中诊断出先天性犬新孢子虫感染，这两个畜群都有流产病史以及新生山羊幼崽体弱的情况。第一只幼崽出生时体弱，起立困难且无法吮乳。可见孔洞脑和脑外积水的肉眼病变。在大脑中观察到多灶性坏死、胶质细胞增生和非化脓性脑炎。在大脑皮层、脑干和小脑中可见几个壁厚的寄生囊肿，这些囊肿仅与犬新孢子虫多克隆抗血清发生强烈反应。第二只幼崽的母亲是犬新孢子虫血清阳性，在上次妊娠中流产。它出生时没有临床症状。新孢子虫病的诊断基于在山羊幼崽摄入初乳前采集的血液通过间接荧光抗体试验获得的犬新孢子虫抗体效价为1:800，并且通过聚合酶链反应从胎盘中检测到犬新孢子虫DNA并进行了测序。这是巴西东南部地区山羊新孢子虫病的首次报告。
