Yang Zhengtao, Wei Zhengkai, Hermosilla Carlos, Taubert Anja, He Xuexiu, Wang Xiaocen, Gong Pengtao, Li Jianhua, Zhang Xichen
College of Basic Medical Sciences, Jilin University, Changchun, China.
College of Veterinary Medicine, Jilin University, Changchun, China.
Front Immunol. 2018 Jan 19;8:2016. doi: 10.3389/fimmu.2017.02016. eCollection 2017.
is an obligate intracellular apicomplexan parasite that causes reproductive loss and severe economic losses in dairy and goat industry. In the present study, we aim to investigate the effects of tachyzoites on the release of extracellular traps (ETs) in caprine monocytes and furthermore elucidated parts of its molecular mechanisms. tachyzoite-induced monocytes-derived ETs formation was detected by scanning electron microscopy. H3 and myeloperoxidase (MPO) within monocyte-ETs structures were examined using laser scanning confocal microscopy analyses. The results showed that tachyzoites were not only able to trigger ETs formation in caprine monocytes, but also that monocyte-released ETs were capable of entrapping viable tachyzoites. Histones and MPO were found to be decorating the DNA within the monocytes derived-ETs structures thus proving the classical components of ETs. Furthermore, inhibitors of NADPH oxidase-, MPO-, ERK 1/2-, or p38 MAPK-signaling pathway significantly decreased tachyzoite-triggered caprine monocyte-derived ETosis. This is the first report of ETs release extruded from caprine monocytes after exposure and thus showing that this early innate immune effector mechanism might be relevant during the acute phase of caprine neosporosis.
是一种专性细胞内顶复门寄生虫，可导致奶牛和山羊养殖业的繁殖损失和严重经济损失。在本研究中，我们旨在研究速殖子对山羊单核细胞中细胞外陷阱（ETs）释放的影响，并进一步阐明其部分分子机制。通过扫描电子显微镜检测速殖子诱导的单核细胞来源的ETs形成。使用激光扫描共聚焦显微镜分析检测单核细胞-ETs结构内的H3和髓过氧化物酶（MPO）。结果表明，速殖子不仅能够触发山羊单核细胞中ETs的形成，而且单核细胞释放的ETs能够捕获活的速殖子。发现组蛋白和MPO修饰单核细胞来源的ETs结构内的DNA，从而证明了ETs的经典成分。此外，NADPH氧化酶、MPO、ERK 1/2或p38 MAPK信号通路的抑制剂显著降低了速殖子触发的山羊单核细胞来源的ETosis。这是首次报道暴露后从山羊单核细胞中挤出ETs的释放，从而表明这种早期先天免疫效应机制可能在山羊新孢子虫病急性期起作用。
