Ragino Yu I, Kashtanova E V, Chernjavski A M, Volkov A M, Polonskaya Ya V, Tsimbal S Yu, Eremenko N V, Ivanova M V
Institute of Therapy, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2011 Jul;151(3):370-3. doi: 10.1007/s10517-011-1333-9.
Blood levels of stem cell marker proteins CD34 and osteonectin were studied in male patients with coronary atherosclerosis by direct biomagnetic separation of proteins with magnetic microspheres using the PureProteome Protein A and Protein G Magnetic Beads proteomic technology. High concentration of osteonectin in the blood was detected, particularly in men with stenosing atherosclerosis and coronary artery calcinosis. Blood osteonectin concentration correlated significantly with some key biomarkers of atherosclerosis and with stenosing atherosclerosis and calcinosis of coronary arteries. The results indicate that osteonectin as a marker of stromal stem cells with osteogenic potential presumably plays an important role in atherogenesis and can serve as a new biomarker of stenosing atherosclerosis and calcinosis of coronary arteries.
通过使用PureProteome蛋白A和蛋白G磁珠蛋白质组学技术，利用磁性微球对蛋白质进行直接生物磁分离，研究了男性冠状动脉粥样硬化患者血液中干细胞标志物蛋白CD34和骨粘连蛋白的水平。检测到血液中骨粘连蛋白浓度较高，尤其是在患有狭窄性动脉粥样硬化和冠状动脉钙化的男性中。血液骨粘连蛋白浓度与动脉粥样硬化的一些关键生物标志物以及冠状动脉狭窄性动脉粥样硬化和钙化显著相关。结果表明，骨粘连蛋白作为具有成骨潜能的基质干细胞的标志物，可能在动脉粥样硬化形成中起重要作用，并且可作为冠状动脉狭窄性动脉粥样硬化和钙化的新生物标志物。
