Smith Kerri M, Xu Yan
Department of Chemistry, Cleveland State University, Cleveland, OH 44115, USA.
Bioanalysis. 2012 Mar;4(6):741-9. doi: 10.4155/bio.12.19.
Analytical quantitation of compounds in tissue has become a more prevalent addition to biological sample analysis in recent times. This increase will most certainly continue to bring the question of proper analytical method validation to the forefront of discussion. Thoughtful design of sample homogenization, analyte fortification and extraction can ensure a successful analysis. This review presents current trends in tissue sample preparation by harvesting, homogenization techniques, as well as concerns for calibrator and QC preparation. Strategies for consideration and resolution of common pitfalls in method development, for example stability issues and control biomatrices in endogenous analysis, are also presented.
近年来，组织中化合物的分析定量已成为生物样品分析中更为普遍的内容。这种增长肯定会继续将合适的分析方法验证问题推到讨论的前沿。精心设计样品匀浆、分析物强化和提取可确保分析成功。本综述介绍了组织样品制备的当前趋势，包括采集、匀浆技术，以及校准物和质量控制制备方面的问题。还介绍了在方法开发中考虑和解决常见陷阱的策略，例如稳定性问题和内源性分析中的对照生物基质。
