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乳腺癌患者中的半胱氨酸组织蛋白酶和基质金属蛋白酶

Cysteine Cathepsins and Matrix Metalloproteases Among Breast Cancer Patients.

作者信息

Tsegaye Solomon, Mehdi Mohammed, Labisso Wajana L, Melka Daniel Seifu

机构信息

Department of Biochemistry, College of Health Sciences, Assela University, Addis Ababa, Ethiopia.

Department of Biochemistry, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.

出版信息

Breast Cancer (Dove Med Press). 2021 May 7;13:271-283. doi: 10.2147/BCTT.S305387. eCollection 2021.

DOI:10.2147/BCTT.S305387
PMID:33994807
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8112858/
Abstract

BACKGROUND

Cellular proteases are thought to increase the likelihood of cancer cell infiltration and metastasis by degrading constituents of the extracellular matrix (ECM). Measuring activities of these proteases may be used as tumor markers for early diagnosis, prognosis, and as a possible target for treatment plan.

OBJECTIVE

The aim of the current study is to evaluate cysteine cathepsins (CTSK and CTSL) and matrix metalloproteases-2 (MMP-2) and 9 (MMP-9) activities in human breast tumor tissue.

METHODS

A comparative cross-sectional study plan was devised to study the enzymatic activities ofCTSK and CTSL andMMP-2 and MMP-9 via zymographic detection method. Sites of tissue sample collection were St Paul's Millennium Medical College, Menelik II Hospital and Zewditu Memorial Hospital, Addis Ababa, Ethiopia. A total of 36 breast cancer patients were recruited and tissue samples were collected for the study.

RESULTS

Activities of CTSK and CTSL were significantly elevated in cancerous tissue than the adjacent normal non-cancerous breast tissue of the same patients (n = 36, p ≤ 0.05). Also, activities ofMMP-2 and MMP-9 were increased significantly in tumor tissues than normal tissues (n = 36, P ≤ 0.05).

CONCLUSION

It is found that there are different patterns of protease enzymatic activity expression between normal and tumor tissue using zymography. Compared with normal tissue samples, the protease enzymatic activity in cancerous tissue is higher. Thus, tissue proteases can be used in conjunction with histological techniques to identify patients in the same clinical group.

摘要

背景

细胞蛋白酶被认为可通过降解细胞外基质（ECM）成分来增加癌细胞浸润和转移的可能性。检测这些蛋白酶的活性可作为肿瘤标志物用于早期诊断、预后评估，并可能作为治疗方案的靶点。

目的

本研究旨在评估人乳腺肿瘤组织中半胱氨酸组织蛋白酶（CTSK和CTSL）以及基质金属蛋白酶-2（MMP-2）和-9（MMP-9）的活性。

方法

设计了一项比较性横断面研究计划，通过酶谱检测法研究CTSK、CTSL以及MMP-2和MMP-9的酶活性。组织样本采集地点为埃塞俄比亚亚的斯亚贝巴的圣保罗千禧医学院、梅内利克二世医院和泽韦迪图纪念医院。共招募了36例乳腺癌患者，并采集组织样本用于研究。

结果

与同一患者的相邻正常非癌乳腺组织相比，癌组织中CTSK和CTSL的活性显著升高（n = 36，p≤0.05）。此外，肿瘤组织中MMP-2和MMP-9的活性也比正常组织显著增加（n = 36，P≤0.05）。

结论

通过酶谱法发现正常组织和肿瘤组织之间存在不同的蛋白酶酶活性表达模式。与正常组织样本相比，癌组织中的蛋白酶酶活性更高。因此，组织蛋白酶可与组织学技术结合使用，以识别同一临床组中的患者。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/10729075c08e/BCTT-13-271-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/e39ee9422b9e/BCTT-13-271-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/457fb985ee2f/BCTT-13-271-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/be0b3a45a9f3/BCTT-13-271-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/8ac8241b3e9d/BCTT-13-271-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/a64c7a6d70ca/BCTT-13-271-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/231d86e7fbc3/BCTT-13-271-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/10729075c08e/BCTT-13-271-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/e39ee9422b9e/BCTT-13-271-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/457fb985ee2f/BCTT-13-271-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/be0b3a45a9f3/BCTT-13-271-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/8ac8241b3e9d/BCTT-13-271-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/a64c7a6d70ca/BCTT-13-271-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/231d86e7fbc3/BCTT-13-271-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/8112858/10729075c08e/BCTT-13-271-g0007.jpg

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