Department of Nutrition and Food Sciences, Physiology and Toxicology, University of Navarra, c/Irunlarrea 1, 31008 Pamplona, Spain.
Exp Biol Med (Maywood). 2012 Apr;237(4):407-16. doi: 10.1258/ebm.2011.011317. Epub 2012 Mar 27.
Antioxidant-based treatments have emerged as novel and interesting approaches to counteract fat accumulation in obesity and associated metabolic disturbances. Adipocytes from rats that were fed on chow or high-fat diet (HFD) for 50 d were isolated (primary adipocytes) and incubated (72 h) on low (LG; 5.6 mmol/L) or high (HG; 25 mmol/L) glucose levels, in the presence or absence of 1.6 nmol/L insulin and 200 μmol/L vitamin C (VC). Adipocytes from HFD-fed animals presented lower insulin-induced glucose uptake, lower lactate and glycerol release, and lower insulin-induced secretion of some adipokines as compared with controls. HG treatment restored the blunted response to insulin regarding apelin secretion in adipocytes from HFD-fed rats. VC treatment inhibited the levels of nearly all variables, irrespective of the adipocytes' dietary origin. The HG treatment reduced adipocyte viability, and VC protected from this toxic effect, although more drastically in control adipocytes. Summing up, in vivo chow or HFD intake determines a differential response to insulin and glucose treatments that appears to be dependent on the insulin-resistance status of the adipocytes, while VC modifies some responses from adipocytes independently of the previous dietary intake of the animals.
抗氧化剂治疗方法作为一种新颖而有趣的方法，已被用于抵抗肥胖症和相关代谢紊乱引起的脂肪堆积。用标准饲料或高脂肪饮食（HFD）喂养 50 天的大鼠的脂肪细胞被分离（原代脂肪细胞），并在存在或不存在 1.6 nmol/L 胰岛素和 200 μmol/L 维生素 C（VC）的情况下，在低（LG；5.6 mmol/L）或高（HG；25 mmol/L）葡萄糖水平下孵育（72 h）。与对照组相比，HFD 喂养的动物的脂肪细胞对胰岛素诱导的葡萄糖摄取、乳酸和甘油释放以及某些脂肪因子的胰岛素诱导分泌的反应较低。HG 处理恢复了 HFD 喂养大鼠脂肪细胞中对脂联素分泌的胰岛素反应迟钝。VC 处理抑制了几乎所有变量的水平，而与脂肪细胞的饮食来源无关。HG 处理降低了脂肪细胞的活力，而 VC 则保护脂肪细胞免受这种毒性作用，尽管在对照组脂肪细胞中更为明显。综上所述，体内摄入标准饲料或 HFD 决定了对胰岛素和葡萄糖处理的不同反应，这种反应似乎依赖于脂肪细胞的胰岛素抵抗状态，而 VC 则独立于动物之前的饮食摄入改变了脂肪细胞的某些反应。
