Molecular Cancer Biology Research Laboratory (MCBRL), Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
Evid Based Complement Alternat Med. 2012;2012:103026. doi: 10.1155/2012/103026. Epub 2012 Feb 6.
With the increased use of plant-based cancer chemotherapy, exploring the antiproliferative effects of phytochemicals for anticancer drug design has gained considerable attention worldwide. This study was undertaken to investigate the effect of walnut green husk extracts on cell proliferation and to determine the possible molecular mechanism of extract-induced cell death by quantifying the expression of Bcl-2, Bax, caspases-3, and Tp53. PC-3 human prostate cancer cells. In this study, we found that green husk extracts suppressed proliferation and induced apoptosis in a dose- and time-dependent manner by modulating expression of apoptosis-related genes. This involved DNA fragmentation (determined by TUNEL assay) and significant changes in levels of mRNA and the expression of corresponding proteins. An increase in expressions of Bax, caspase-3, and tp53 genes and their corresponding proteins was detected using real-time PCR and western blot analysis in PC-3 cells treated with the green husk organic extracts. In contrast, Bcl2 expression was downregulated after exposure to the extracts. Our data suggest the presence of bioactive compound(s) in walnut green husks that are capable of killing prostate carcinoma cells by inducing apoptosis and that the husks are a candidate source of anticancer drugs.
随着植物来源的癌症化疗药物的广泛应用，探索植物化学物质的抗增殖作用以用于抗癌药物设计已引起全球的广泛关注。本研究旨在探讨山核桃绿皮提取物对细胞增殖的影响，并通过定量分析 Bcl-2、Bax、caspase-3 和 Tp53 的表达，确定提取物诱导细胞死亡的可能分子机制。选用 PC-3 人前列腺癌细胞。在这项研究中，我们发现绿皮提取物通过调节凋亡相关基因的表达，以剂量和时间依赖的方式抑制增殖并诱导细胞凋亡。这涉及到 DNA 片段化（通过 TUNEL 测定确定）以及 mRNA 水平和相应蛋白表达的显著变化。用实时 PCR 和 Western blot 分析检测到用绿皮有机提取物处理的 PC-3 细胞中 Bax、caspase-3 和 tp53 基因及其相应蛋白的表达增加。相比之下，暴露于提取物后 Bcl2 的表达下调。我们的数据表明山核桃绿皮中存在具有生物活性的化合物，这些化合物能够通过诱导细胞凋亡杀死前列腺癌细胞，并且山核桃绿皮是抗癌药物的候选来源。
