Tissue Engineering & Reparative Dentistry, Cardiff Institute of Tissue Engineering & Repair-CITER, School of Dentistry, Cardiff University, Cardiff, UK.
J Biomater Appl. 2013 Jul;28(1):144-60. doi: 10.1177/0885328212441495. Epub 2012 Mar 28.
This study investigated the effects of combined titanium nano-/micron-scale roughness, induced by hydrogen peroxide pre-treatments, on bone marrow stromal cell responses and Porphyromonas gingivalis adherence in vitro. Untreated surfaces exhibited nano-scale features, while hydrogen peroxide treatments promoted increased nano-/micron-scale roughness. Bone marrow stromal cell attachment and proliferation were maintained with 6 h and 24 h treatments, but significantly decreased on 1-week and 4-week-treated surfaces. Bone marrow stromal cells on 6 h-4 week-treated titanium demonstrated enhanced osteogenic differentiation versus untreated surfaces. P. gingivalis adherence was significantly increased on 24 h-4 week surfaces. Results suggest that 6 h but less than 24 h treatments maintain or promote bone marrow stromal cell responses while minimizing microbial adherence, potentially enhancing titanium surface bio-activation for osseointegration.
本研究探讨了经过氧化氢预处理诱导的钛纳米/微米复合粗糙度对骨髓基质细胞反应和牙龈卟啉单胞菌黏附的体外影响。未经处理的表面呈现纳米级特征，而过氧化氢处理则促进了纳米/微米复合粗糙度的增加。骨髓基质细胞的黏附和增殖在 6 小时和 24 小时处理后得以维持，但在 1 周和 4 周处理后显著减少。与未经处理的表面相比，在 6 小时-4 周处理的钛表面上，骨髓基质细胞表现出增强的成骨分化。在 24 小时-4 周表面上，牙龈卟啉单胞菌的黏附显著增加。结果表明，6 小时但短于 24 小时的处理维持或促进了骨髓基质细胞的反应，同时最小化了微生物的黏附，可能增强了钛表面的生物活性以实现骨整合。
