Division of Pulmonology, Allergy and Critical Care Medicine, Department of Internal Medicine, Chungnam National University Medical School, Daesadong, Daejeon, South Korea.
Int J Nanomedicine. 2012;7:1329-43. doi: 10.2147/IJN.S27159. Epub 2012 Mar 14.
The anti-inflammatory action of silver nanoparticles (NPs) has been reported in a murine model of asthma in a previous study. But more specific mechanisms of silver NPs in an attenuation of allergic airway inflammation have not yet been established. Vascular and mucous changes are believed to contribute largely in pathophysiology in asthma. Among various factors related to vascular changes, vascular endothelial growth factor (VEGF) plays a pivotal role in vascular changes in asthma. Mucin proteins MUC5AC and MUC5B have been implicated as markers of goblet cell metaplasia in lung pathologies. The aim of this study was to investigate the effects of silver NPs on VEGF signaling pathways and mucus hypersecretion. Ovalbumin (OVA)-inhaled female BALBc mice were used to evaluate the role of silver NPs and the related molecular mechanisms in allergic airway disease. In this study, with an OVA-induced murine model of allergic airway disease, it was found that the increased levels of hypoxia-inducible factor (HIF)-1α, VEGF, phosphatidylinositol-3 kinase (PI3K) and phosphorylated-Akt levels, and mucous glycoprotein expression (Muc5ac) in lung tissues were substantially decreased by the administration of silver NPs. In summary, silver NPs substantially suppressed mucus hypersecretion and PI3K/HIF-1α/VEGF signaling pathway in an allergic airway inflammation.
先前的一项研究报道了纳米银（NPs）在哮喘小鼠模型中的抗炎作用。但是，纳米银在减轻过敏气道炎症方面的更具体机制尚未确定。血管和黏液变化被认为在哮喘的病理生理学中起主要作用。在与血管变化相关的各种因素中，血管内皮生长因子（VEGF）在哮喘中的血管变化中起着关键作用。黏蛋白蛋白 MUC5AC 和 MUC5B 被认为是肺病理学中杯状细胞化生的标志物。本研究旨在探讨纳米银对 VEGF 信号通路和黏液高分泌的影响。卵清蛋白（OVA）吸入的雌性 BALBc 小鼠用于评估纳米银在过敏性气道疾病中的作用及其相关的分子机制。在这项研究中，通过 OVA 诱导的过敏性气道疾病小鼠模型，发现给予纳米银后，肺组织中缺氧诱导因子（HIF）-1α、VEGF、磷酸肌醇-3 激酶（PI3K）和磷酸化-Akt 水平以及黏蛋白糖蛋白表达（Muc5ac）的升高水平显著降低。综上所述，纳米银在过敏性气道炎症中显著抑制了黏液高分泌和 PI3K/HIF-1α/VEGF 信号通路。
