CIGMH/DCV, Faculdade de Ciências e Tecnologia - Universidade Nova de Lisboa, Campus da Caparica, 2829-516 Caparica, Portugal.
J Proteomics. 2012 Jun 6;75(10):2811-23. doi: 10.1016/j.jprot.2011.11.007. Epub 2011 Nov 17.
Nanotechnology has prompted researchers to develop new and improved materials aimed at biomedical applications with particular emphasis in diagnostics and therapy. Special interest has been directed at providing enhanced biomolecular diagnostics, including SNP detection gene expression profiles and biomarker characterisation. These strategies have focused on the development of nanoscale devices and platforms that can be used for single molecule characterisation of nucleic acid, DNA or RNA, and protein at an increased rate when compared to traditional techniques. Also, several advances have been reported on DNA analysis in real time, at both high resolution and very high throughputs, suitable for biomedical diagnostics. Here, we shall provide a review of available nanotechnology-based platforms for biomolecular recognition, and their application to molecular diagnostics and genome analysis, with emphasis on the use of noble metal nanoparticles for simple and specific analysis systems. Particular focus will be put on those already being translated into clinical settings. This article is part of a Special Issue entitled: Proteomics: The clinical link.
纳米技术促使研究人员开发新的和改进的材料，旨在将其应用于生物医学领域，尤其侧重于诊断和治疗。特别关注的是提供增强的生物分子诊断，包括 SNP 检测、基因表达谱和生物标志物特征。这些策略集中于开发纳米级设备和平台，这些设备和平台可用于单分子核酸、DNA 或 RNA 以及蛋白质的特征化，与传统技术相比，其具有更高的速度。此外，据报道，在高分辨率和非常高的高通量下，实时 DNA 分析已经取得了几项进展，适用于生物医学诊断。在这里，我们将对现有的基于纳米技术的生物分子识别平台进行综述，并将其应用于分子诊断和基因组分析，重点是使用贵金属纳米粒子用于简单和特定的分析系统。特别关注的是那些已经转化为临床应用的系统。本文是一个特刊的一部分，该特刊的主题是：蛋白质组学：临床联系。
