Zhao Yanan, Liu Yiming, Shan Jiheng, Xu Xiaohong, Zhang Chengzhi, Liu Zaoqu, Li Xiaomeng, Zhong Zhaoqian, Gao Yanxia, Ren Kewei, Jiao Dechao, Ren Jianzhuang, Wu Ping, Jiang Yong, Han Xinwei
Department of Interventional Radiology, Key Laboratory of Interventional Radiology of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450001, China.
Interventional Institute of Zhengzhou University, Zhengzhou, 450001, China.
J Nanobiotechnology. 2025 Jan 29;23(1):59. doi: 10.1186/s12951-024-03087-y.
Excessive vascularization during tracheal in-stent restenosis (TISR) is a significant but frequently overlooked issue. We developed an anti-inflammatory coupled anti-angiogenic airway stent (PAGL) incorporating anlotinib hydrochloride and silver nanoparticles using advanced electrospinning technology. PAGL exhibited hydrophobic surface properties, exceptional mechanical strength, and appropriate drug-release kinetics. Moreover, it demonstrated a remarkable eradication effect against methicillin-resistant Staphylococcus aureus. It also displayed anti-proliferative and anti-angiogenic properties on human umbilical vein endothelial cells and lung fibroblasts. PAGL was implanted into the tracheae of New Zealand rabbits to evaluate its efficacy in inhibiting bacterial infection, suppressing the inflammatory response, reducing angiogenesis, and attenuating excessive fibroblast activation. RNA sequencing analysis revealed a significant downregulation of genes associated with fibrosis, intimal hyperplasia, and cell migration following PAGL treatment. This study provides insight into the development of airway stents that target angiogenesis and inflammation to address problems associated with TISR effectively and have the potential for clinical translation.
气管内支架再狭窄（TISR）期间的过度血管生成是一个重要但经常被忽视的问题。我们利用先进的静电纺丝技术开发了一种结合盐酸安罗替尼和银纳米颗粒的抗炎联合抗血管生成气道支架（PAGL）。PAGL具有疏水表面特性、出色的机械强度和适当的药物释放动力学。此外，它对耐甲氧西林金黄色葡萄球菌显示出显著的根除效果。它还对人脐静脉内皮细胞和肺成纤维细胞表现出抗增殖和抗血管生成特性。将PAGL植入新西兰兔的气管中，以评估其在抑制细菌感染、抑制炎症反应、减少血管生成和减轻成纤维细胞过度活化方面的疗效。RNA测序分析显示，PAGL治疗后与纤维化、内膜增生和细胞迁移相关的基因显著下调。本研究为开发针对血管生成和炎症的气道支架提供了见解，以有效解决与TISR相关的问题，并具有临床转化潜力。
