Armour J C, Lucisano J Y, McKean B D, Gough D A
Department of Applied Mechanics and Engineering Sciences, University of California, San Diego, La Jolla 92093.
Diabetes. 1990 Dec;39(12):1519-26. doi: 10.2337/diab.39.12.1519.
An intravenous glucose sensor was implanted in six dogs for 1-15 wk. The glucose sensor is a flexible cylinder, approximately 0.2 cm diam and 30 cm long, with a tip containing immobilized glucose oxidase and catalase coupled to a potentiostatic O2 sensor. The sensor and a similar O2 reference sensor were implanted in the superior vena cava near the entrance of the right atrium. The sensor response was conveyed externally either by a telemetry system implanted nearby, surgically accessed leads, or chronically maintained percutaneous leads. Summing over the six implants, there was a total implantation period of 333 days during which glucose sensors were functional on demand. The sensor response showed agreement with conventionally assayed blood samples after accounting for a response lag. Sensor response to glucose showed little change over the implant period. Biocompatibility, enzyme lifetime, O2 availability, O2 sensor stability, and biochemical interference were not limitations. Results demonstrated that this sensor can function effectively as an implant in dogs for a period of months and has the potential for long-term operation.
将一个静脉葡萄糖传感器植入6只狗体内1至15周。该葡萄糖传感器是一个柔性圆柱体，直径约0.2厘米，长30厘米，其尖端含有固定化葡萄糖氧化酶和与恒电位O2传感器相连的过氧化氢酶。传感器和一个类似的O2参比传感器被植入右心房入口附近的上腔静脉。传感器的响应通过附近植入的遥测系统、手术接入的导线或长期维持的经皮导线从外部进行传输。在这6次植入实验中，葡萄糖传感器能够按需正常工作的总植入期为333天。在考虑了响应延迟后，传感器的响应与传统检测的血样结果相符。在植入期间，传感器对葡萄糖的响应几乎没有变化。生物相容性、酶寿命、O2可用性、O2传感器稳定性和生化干扰均不存在限制因素。结果表明，这种传感器在狗体内作为植入物可有效工作数月，具有长期运行的潜力。
