Environmental Engineering Department, Istanbul Technical University, 34469 Maslak, Istanbul, Turkey.
Bioresour Technol. 2012 Jun;114:109-16. doi: 10.1016/j.biortech.2012.03.020. Epub 2012 Mar 10.
The study evaluated the short-term inhibition impact of three antimicrobials, sulfamethoxazole, erythromycin and tetracycline, on the methanogenic activity of acclimated biomass fed with acetate. Batch reactors were inoculated each with a different antimicrobial concentration in the range of 1-1000 mg/L and they were operated during 6 days. Organic substrate removal was monitored by both soluble COD and acetate measurements, together with daily measurements of biogas and methane generation. While acetate was almost fully removed in all experiments, methane generation exhibited a significant drop with increasing antimicrobial doses. Almost complete methane inhibition was observed for antimicrobial doses above 500 mg/L. Together with adverse impact on process kinetics in the early phases of the experiments, the final acute impact of antimicrobials was on process stoichiometry, preventing complete utilization of acetate removed in metabolic reactions. The observed effect was found compatible with uncompetitive inhibition, which similarly exerts a binding impact on substrate-enzyme complex.
本研究评估了三种抗生素（磺胺甲噁唑、红霉素和四环素）对乙酸驯化生物量产甲烷活性的短期抑制影响。在 1-1000mg/L 的范围内，每个批次反应器接种不同浓度的抗生素，并运行 6 天。通过可溶性 COD 和乙酸测量以及每日沼气和甲烷生成测量来监测有机底物去除。虽然所有实验中乙酸几乎都被完全去除，但随着抗生素剂量的增加，甲烷生成量显著下降。当抗生素剂量超过 500mg/L 时，几乎完全抑制了甲烷生成。除了对实验早期阶段过程动力学的不利影响外，抗生素的最终急性影响还在于过程化学计量学，阻止了代谢反应中去除的乙酸的完全利用。观察到的效果与非竞争性抑制一致，后者同样对底物-酶复合物产生结合影响。
