Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807 Taiwan, ROC.
Phytochemistry. 2012 Jun;78:120-5. doi: 10.1016/j.phytochem.2012.02.023. Epub 2012 Mar 27.
Bioassay-guided fractionation of the roots of Anneslea fragrans var. lanceolata led to the isolation of four dihydrochalcone glucosides, davidigenin-2'-O-(6″-O-4″'-hydroxybenzoyl)-β-glucoside (1), davidigenin-2'-O-(2″-O-4″'-hydroxybenzoyl)-β-glucoside (2), davidigenin-2'-O-(3″-O-4″'-hydroxybenzoyl)-β-glucoside (3), and davidigenin-2'-O-(6″-O-syringoyl)-β-glucoside (4), and 13 known compounds. The structures were identified by means of spectroscopic analysis. Davidigenin-2'-O-(6″-O-syringoyl)-β-glucoside (4), 1-O-3,4-dimethoxy-5-hydroxyphenyl-6-O-(3,5-di-O-methylgalloyl)-β-glucopyranoside (5), lyoniresinol (10), and syringic acid (13) showed ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)] cation radical scavenging activity, with SC(50) values of 52.6 ± 5.5, 26.0 ± 0.7, 6.0 ± 0.2, and 27.5 ± 0.6 μg/mL in 20 min, respectively. Lyoniresinol (10), isofraxidin (12), and syringic acid (13) also showed DPPH [1,1-diphenyl-2-picrylhydrazyl] radical scavenging activity, with SC(50) values of 8.4 ± 1.8, 51.6 ± 2.2, and 4.3 ± 0.7 μg/mL in 30 min, respectively.
基于生物活性的导向分离，从清香藤根部分离得到 4 个二氢查尔酮葡萄糖苷，分别鉴定为：大卫酮-2'-O-(6″-O-4″'-羟基苯甲酰基)-β-葡萄糖苷（1）、大卫酮-2'-O-(2″-O-4″'-羟基苯甲酰基)-β-葡萄糖苷（2）、大卫酮-2'-O-(3″-O-4″'-羟基苯甲酰基)-β-葡萄糖苷（3）和大卫酮-2'-O-(6″-O-丁香酰基)-β-葡萄糖苷（4），以及 13 个已知化合物。结构通过光谱分析鉴定。大卫酮-2'-O-(6″-O-丁香酰基)-β-葡萄糖苷（4）、1-O-3,4-二甲氧基-5-羟基苯基-6-O-(3,5-二-O-甲基没食子酰基)-β-吡喃葡萄糖苷（5）、莱苏醇（10）和丁香酸（13）表现出 ABTS [2,2'- 偶氮-双(3-乙基苯并噻唑啉-6-磺酸)]阳离子自由基清除活性，在 20 分钟时的 SC(50)值分别为 52.6±5.5、26.0±0.7、6.0±0.2 和 27.5±0.6μg/mL。莱苏醇（10）、异落叶松脂素（12）和丁香酸（13）也表现出 DPPH [1,1-二苯基-2-苦基肼]自由基清除活性，在 30 分钟时的 SC(50)值分别为 8.4±1.8、51.6±2.2 和 4.3±0.7μg/mL。
