葡萄多酚-豆粉复合物的生化分析及体内降血糖活性
Biochemical analysis and in vivo hypoglycemic activity of a grape polyphenol-soybean flour complex.
机构信息
School of Environmental and Biological Sciences, Foran Hall, Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, New Jersey 08901, USA.
出版信息
J Agric Food Chem. 2012 Sep 12;60(36):8860-5. doi: 10.1021/jf300232h. Epub 2012 Apr 16.
Abstract
Defatted soybean flour (DSF) can efficiently sorb, concentrate, and stabilize polyphenols, but not sugars, from Concord grape juice, to yield grape polyphenol-enriched DSF. Sorption of grape polyphenols to DSF particles was dependent on the ratio of DSF and grape juice concentrate used, but not time of mixing or pH. Depending on ratios of starting materials, 1 g of grape polyphenol-enriched DSF contained 1.6-10.4 mg of anthocyanins, 7.5-93.1 mg of proanthocyanidins, and 20.5-144.5 mg of total polyphenols. LC-MS analysis of grape juice samples before and after addition and removal of DSF and eluate from grape polyphenol-enriched DSF confirmed that a broad range of grape compounds were sorbed to the DSF matrix. Finally, grape polyphenol-enriched DSF was able to significantly lower blood glucose levels in hyperglycemic C57BL/6J mice. The data indicate that grape polyphenol-enriched DSF can provide a high-protein, low-sugar ingredient for delivery of concentrated grape polyphenolics.
摘要
脱脂大豆粉（DSF）可以有效地吸附、浓缩和稳定葡萄汁中的多酚，但不能吸附其中的糖，从而得到富含多酚的 DSF。DSF 颗粒对葡萄多酚的吸附取决于 DSF 和葡萄汁浓缩物的比例，但与混合时间或 pH 值无关。根据起始材料的比例，1 克富含葡萄多酚的 DSF 含有 1.6-10.4 毫克花色苷、7.5-93.1 毫克原花青素和 20.5-144.5 毫克总多酚。通过 LC-MS 分析添加和去除 DSF 前后以及从富含葡萄多酚的 DSF 洗脱液中的葡萄汁样品，证实了广泛的葡萄化合物被吸附到 DSF 基质上。最后，富含葡萄多酚的 DSF 能够显著降低高血糖 C57BL/6J 小鼠的血糖水平。数据表明，富含葡萄多酚的 DSF 可以为提供高蛋白、低糖的浓缩葡萄多酚成分。
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