Moghadasi Negar, Andisheh-Tadbir Azadeh, Samiee Amin, Torabi Ardekani Shima, Khademi Bijan, Malekzadeh Mahyar, Zare Razieh
Undergraduate Student, School of Dentistry, International Branch, Shiraz University of Medical Sciences, Shiraz, Iran.
Oral and Dental Disease Research Center, Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
J Dent (Shiraz). 2022 Sep;23(3):251-256. doi: 10.30476/DENTJODS.2021.89066.1384.
Heat shock protein 27 (HSP) plays important roles in many cellular processes and has been implicated in different types of diseases such as cancers.
This study aimed to evaluate the serum level of HSP in patients with salivary gland tumors and to determine its possible correlation with the prognosis of the disease.
This cross-sectional study was performed on 60 patients with sali-vary gland tumor including 16 pleomorphic adenoma, 33 adenoid cystic carcinoma, 6 mu-coepidermoid carcinoma, 5 acinic cell carcinoma, and 28 healthy control subjects. The con-trol cases were healthy blood donors who matched the study group in age and sex. Serum samples were obtained from the clotted blood and HSPconcentrations were measured with sandwich enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed by using one-way ANOVA, post Hoc test, independent sample t-test, and ROC analysis. A p value of less than 0.05 was considered as significant.
The mean serum level of HSP was 3956.1±3830.1 (pg/ml) in patients with malig-nant salivary gland tumor, which was significantly higher than that in benign salivary gland tumor (752.2±485.6) and healthy controls (602.3±575.8) ( <0.001). However, there was no significant difference in the HSP serum levels between the patients with benign salivary gland tumors and healthy controls (= 0.2). No association was detected between the mean serum levels of HSP and clinicopathologic factors such as age, sex, stage and nodal metas-tasis ( > 0.05), except for the tumor size (= 0.04).
The HSP concentration increased in patients with malignant salivary gland tumors. Moreover, the HSP level was correlated with tumor growth, invasiveness, and diagnosability. Yet, larger clinical studies are required to explore its prognostic value.
热休克蛋白27（HSP）在许多细胞过程中发挥重要作用，并与多种疾病如癌症有关。
本研究旨在评估涎腺肿瘤患者血清中HSP水平，并确定其与疾病预后的可能相关性。
本横断面研究对60例涎腺肿瘤患者进行，包括16例多形性腺瘤、33例腺样囊性癌、6例黏液表皮样癌、5例腺泡细胞癌，以及28例健康对照者。对照病例为年龄和性别与研究组匹配的健康献血者。从凝血样本中获取血清样本，采用夹心酶联免疫吸附测定（ELISA）法测定HSP浓度。采用单因素方差分析、事后检验、独立样本t检验和ROC分析进行统计学分析。p值小于0.05被认为具有统计学意义。
恶性涎腺肿瘤患者血清HSP平均水平为3956.1±3830.1（pg/ml），显著高于良性涎腺肿瘤患者（752.2±485.6）和健康对照者（602.3±575.8）（<0.001）。然而，良性涎腺肿瘤患者与健康对照者的血清HSP水平无显著差异（=0.2）。除肿瘤大小外（=0.04），未检测到HSP平均血清水平与年龄、性别、分期和淋巴结转移等临床病理因素之间存在关联（>0.05）。
恶性涎腺肿瘤患者HSP浓度升高。此外，HSP水平与肿瘤生长、侵袭性和可诊断性相关。然而，需要更大规模的临床研究来探讨其预后价值。
