采用多重逆转录聚合酶链反应鉴定病毒性嗅觉功能障碍患者的病毒。
Identification of Viruses in Patients With Postviral Olfactory Dysfunction by Multiplex Reverse-Transcription Polymerase Chain Reaction.
机构信息
Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Section of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Chicago, Chicago, Illinois, U.S.A.
出版信息
Laryngoscope. 2021 Jan;131(1):158-164. doi: 10.1002/lary.28997. Epub 2020 Aug 12.
OBJECTIVES/HYPOTHESIS: To investigate causative viruses in patients with postviral olfactory disorders (PVOD).
STUDY DESIGN
Case-control study.
METHODS
One hundred fifty-one consecutive patients diagnosed with PVOD were enrolled, and samples from 38 patients who visited the doctor within 3 months of symptom onset were collected and analyzed. Thirty-two individuals who underwent surgery for nasal septal deviation during the same time period were collected as the control group. The Sniffin' Sticks psychophysical olfactory test was used to evaluate olfactory function. Olfactory cleft specimens were collected using nasopharyngeal flocked swabs (COPAN FLOQSwabs). Eighteen viruses were tested for with the Luminex xTAG RVP FAST v2 Assay Kit.
RESULTS
Out of the 38 patients with PVOD, rhinoviruses were detected in 13 patients, and coronavirus OC43 was detected in one patient. The frequency of positive virus detection in the patients with anosmia was higher than in those with hyposmia (58.8% vs. 19.0%, P = 0.018). In control group, rhinovirus was identified in one patient (3.1%). Nasal obstruction was the most common symptom and was experienced by 71.0% of patients.
CONCLUSIONS
Rhinovirus and coronavirus are more commonly identified in PVOD. Our methods represent an approach to screen for viruses that may be involved in PVOD.
LEVEL OF EVIDENCE
4 Laryngoscope, 131:158-164, 2021.
目的/假设：研究病毒性嗅觉障碍（PVOD）患者的致病病毒。
研究设计
病例对照研究。
方法
纳入 151 例连续确诊为 PVOD 的患者，采集并分析 38 例症状发作后 3 个月内就诊的患者样本。同期因鼻中隔偏曲接受手术的 32 名个体作为对照组。采用嗅敏度测试 Sniffin' Sticks 评估嗅觉功能。使用鼻咽拭子（COPAN FLOQSwabs）采集嗅裂标本。采用 Luminex xTAG RVP FAST v2 检测试剂盒检测 18 种病毒。
结果
38 例 PVOD 患者中，13 例检测到鼻病毒，1 例检测到冠状病毒 OC43。无嗅觉患者的病毒阳性检出率高于嗅觉减退患者（58.8% vs. 19.0%，P = 0.018）。对照组中，1 例患者（3.1%）检测到鼻病毒。鼻塞是最常见的症状，71.0%的患者有此症状。
结论
鼻病毒和冠状病毒在 PVOD 中更为常见。我们的方法代表了一种筛查可能与 PVOD 相关病毒的方法。
证据等级
4 Laryngoscope, 131:158-164, 2021.
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