The Department of Human Genetics, New York State Institute for Basic Research in Developmental Disabilities-IBR, 1050 Forest Hill Road, Staten Island, NY 10314, USA.
Cryobiology. 2012 Aug;65(1):72-3. doi: 10.1016/j.cryobiol.2012.03.005. Epub 2012 Mar 29.
We have observed evidence of increased telomere shortening in short-term T-lymphocyte cultures following freezing and thawing of the original inoculum obtained by ficoll-paque gradient centrifugation, compared to T-lymphocytes that were cultured immediately without freezing and thawing from the same blood sample from 3 female and 3 male adults. Because freezing may have similar effects on other cell types, and because telomere shortening may only manifest its effects after many years or decades, we suggest there is a pressing need for evaluation of the effects of freezing on any cells envisioned for clinical applications, including embryo implantation.
我们观察到，与直接从 3 名女性和 3 名男性成年人的同一血样培养而未经冷冻和解冻的 T 淋巴细胞相比，通过菲可-帕奎梯度离心获得的原始接种物的短期 T 淋巴细胞培养物在冷冻和解冻后端粒缩短的证据增加。由于冷冻可能对其他细胞类型产生类似的影响，并且端粒缩短可能仅在多年或数十年后才表现出其影响，因此我们建议迫切需要评估冷冻对任何预期用于临床应用的细胞（包括胚胎植入）的影响。
