Kim Hyun-Jeong, Park Jun-Gyu, Alfajaro Mia Madel, Kim Deok-Song, Hosmillo Myra, Son Kyu-Yeol, Lee Ju-Hwan, Bae You-Chan, Park Sang-Ik, Kang Mun-Il, Cho Kyoung-Oh
Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
Vet Microbiol. 2012 Sep 14;159(1-2):11-22. doi: 10.1016/j.vetmic.2012.03.017. Epub 2012 Mar 16.
Rotaviruses are important human and animal pathogens with high impact on public health and livestock industry. There is little evidence about the cross-species pathogenicity and extra-intestinal infections of animal and human reassortant rotaviruses, particularly based on all 11 genotyping data. In this study, the bovine triple reassortant KJ56-1 strain harboring two bovine-like genome segments, eight porcine-like genome segments, and one human-like genome segment was used to evaluate the cross-species pathogenicity in its parent species, calves and piglets, and to determine its abilities of causing viremia and extra-intestinal tropisms in piglets. The KJ56-1 strain isolated from a calf diarrhea fecal sample replicated without causing diarrhea and severe intestinal pathology in calves. However, piglets inoculated with this strain showed persistent severe diarrhea and marked intestinal pathology. By SYBR Green real-time RT-PCR, viral RNA was detected in the sera, mesenteric lymph node, lung, liver, choroid plexus, and cerebrospinal fluid in the experimental piglets. An immunofluorescence assay confirmed viral replication in these extra-intestinal organs and tissues. These results indicated that the bovine triple reassortant KJ56-1 strain was virulent to piglets but not to calves. Our data also demonstrated that the reassortant rotaviruses had the ability to spread to the bloodstream from the gut, enter and amplify in the mesenteric lymph node, and disseminate to the extra-intestinal organs and tissues.
轮状病毒是对人类和动物都很重要的病原体，对公共卫生和畜牧业有重大影响。关于动物和人类重配轮状病毒的跨物种致病性和肠外感染，尤其是基于所有11个基因分型数据的相关证据很少。在本研究中，使用携带两个牛样基因组片段、八个猪样基因组片段和一个人样基因组片段的牛三重重配KJ56-1毒株，评估其在亲本物种（犊牛和仔猪）中的跨物种致病性，并确定其在仔猪中引起病毒血症和肠外嗜性的能力。从一头犊牛腹泻粪便样本中分离出的KJ56-1毒株在犊牛中复制，但未引起腹泻和严重的肠道病变。然而，接种该毒株的仔猪出现持续严重腹泻和明显的肠道病变。通过SYBR Green实时RT-PCR，在实验仔猪的血清、肠系膜淋巴结、肺、肝、脉络丛和脑脊液中检测到病毒RNA。免疫荧光分析证实了该病毒在这些肠外器官和组织中的复制。这些结果表明，牛三重重配KJ56-1毒株对仔猪具有致病性，但对犊牛无致病性。我们的数据还表明，重配轮状病毒有能力从肠道扩散到血液中，进入肠系膜淋巴结并在其中扩增，然后传播到肠外器官和组织。
