Dubal Zunjar Baburao, Bhilegaonkar Kiran N, Barbuddhe Sukhadeo B, Kolhe Rahul P, Kaur Simranpreet, Rawat Shriya, Nambiar Prejit, Karunakaran Muthu
Animal Science Section, ICAR Research Complex for Goa, Old Goa, 403402, Goa, India.
Trop Anim Health Prod. 2013 Feb;45(2):609-15. doi: 10.1007/s11250-012-0267-1. Epub 2012 Sep 26.
Group A rotaviruses can infect both humans and animals and have been recognized as an important cause of diarrhea in porcine. In this study, we report the prevalence and molecular epidemiology of rotaviruses detected in piglets in different regions of India. A total 275 fecal samples (180 diarrheal and 95 non-diarrheal) from piglets were collected from the western (135), southern (60), northern (20), and North-Eastern Hill (NEH) (60) regions of India and tested for rotaviruses. All the samples were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reverse transcription-polymerase chain reaction (RT-PCR). Rotaviruses were detected in 10.18 % of samples by SDS-PAGE and/or RT-PCR with a maximum of 30 % from the NEH region followed by 7.4 % from the western region. Samples from the southern and northern regions were found to be negative. Only 10 isolates were subjected to genotypic characterization using amplification of VP7 and VP4 genes followed by two separate multiplex PCR assays for G genotyping and another two for P genotyping using genotype-specific primers. Of these, three isolates could be typed as G4 specificity, one with G9, and three as P[6] leading to identification of an uncommon strain, G4P[6]. One isolate was further confirmed by nucleotide sequencing. The data demonstrate genetic diversity of porcine rotavirus strains and suggest that pig farms may serve as potential reservoirs for human infections.
A组轮状病毒可感染人类和动物，已被确认为猪腹泻的重要病因。在本研究中，我们报告了在印度不同地区仔猪中检测到的轮状病毒的流行情况和分子流行病学。从印度西部（135份）、南部（60份）、北部（20份）和东北山区（NEH）（60份）收集了总共275份仔猪粪便样本（180份腹泻样本和95份非腹泻样本），并检测轮状病毒。所有样本均进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和逆转录-聚合酶链反应（RT-PCR）。通过SDS-PAGE和/或RT-PCR在10.18%的样本中检测到轮状病毒，其中NEH地区的检出率最高，为30%，其次是西部地区，为7.4%。发现南部和北部地区的样本为阴性。仅对10株分离株进行了基因型鉴定，方法是扩增VP7和VP4基因，然后分别使用两种多重PCR检测进行G基因型分型，另外两种使用基因型特异性引物进行P基因型分型。其中，三株分离株可分型为G4特异性，一株为G9，三株为P[6]，从而鉴定出一种罕见毒株G4P[6]。一株分离株通过核苷酸测序得到进一步证实。数据表明猪轮状病毒株具有遗传多样性，并提示猪场可能是人类感染的潜在储存宿主。
