Chen Mei, Tian Li-Li, Shi Qing-Hui, Cao Tian-Wen, Hao Jia-Sheng
Anhui Normal University, Wuhu Anhui, China.
Dongwuxue Yanjiu. 2012 Apr;33(2):191-201. doi: 10.3724/SP.J.1141.2012.02191.
The complete mitochondrial genome of Apatura ilia (GenBank accession no. JF437925) was determined as a circular DNA molecule of 15 242 bp, with common genes of 13 putative proteins, 2 rRNAs, and 22 tRNAs and of the same gene arrangement as in other sequenced lepidopterans. All protein-coding genes had the typical start codon ATN, except for the COI's using CGA as its start codon as previously demonstrated in other lepidopteran species. The comparison of the nucleotide sequences of the A. ilia mitogenome with ten other Nymphalidae species showed nearly identical gene orientation and arrangement, with only a few alterations in non-coding fragments. The nucleotide composition and codon frequency all fell into the range estimated for the order Lepidoptera. The A. ilia mitochondrial genome had the canonical set of 22 tRNA genes folded in the typical cloverleaf structure, with an unique exception of tRNA(Ser) (AGN). The mitochondrial genes from A. ilia were overlapped in a total of 33 bp at 9 locations, as well as interleaved with a total of 155 bp intergenic spacers, spread over 12 regions with the size ranging from 1 to 49 bp. Furthermore, the spacer between ND6 and Cyt b harbored a microsatellite-like repeat (TA)(23) not found in other completely sequenced nymphalid genomes. The 403 bp AT-rich region harbored two conserved motifs (ATAGA, ATTTA), a 21 bp polyT stretch, a 10 bp poly-A region, along with two microsatellite-like repeats ( (TA)(10) and (TA)(7)), as detected in other nymphalid butterflies.
测定了柳闪蝶（Apatura ilia）的完整线粒体基因组（GenBank登录号：JF437925），其为一个15242 bp的环状DNA分子，含有13个推定蛋白质、2个rRNA和22个tRNA的常见基因，且基因排列与其他已测序鳞翅目昆虫相同。所有蛋白质编码基因都有典型的起始密码子ATN，但细胞色素氧化酶亚基I（COI）使用CGA作为起始密码子，这与之前在其他鳞翅目物种中观察到的情况一致。将柳闪蝶线粒体基因组的核苷酸序列与其他十种蛱蝶科物种进行比较，结果显示基因方向和排列几乎相同，仅非编码片段有一些变化。核苷酸组成和密码子频率均落在鳞翅目估计的范围内。柳闪蝶线粒体基因组有一套标准的22个tRNA基因，折叠成典型的三叶草结构，唯一例外的是tRNA(Ser) (AGN)。柳闪蝶的线粒体基因在9个位置共有33 bp的重叠，还有总共155 bp的基因间隔区，分布在12个区域，大小从1到49 bp不等。此外，ND6和细胞色素b之间的间隔区含有一个微卫星样重复序列（TA）(23)，这在其他完全测序的蛱蝶科基因组中未发现。在403 bp的富含AT区域含有两个保守基序（ATAGA、ATTTA）、一个21 bp的多聚T序列、一个10 bp的多聚A区域，以及两个微卫星样重复序列（(TA)(10)和(TA)(7)），这与在其他蛱蝶科蝴蝶中检测到的情况相同。
