Department of Morphology and Genetics, Laboratory of Developmental Biology, Federal University of Sao Paulo, Sao Paulo, Brazil.
Oral Dis. 2012 Oct;18(7):648-54. doi: 10.1111/j.1601-0825.2012.01917.x. Epub 2012 Apr 4.
Infective agents may affect pregnancy outcomes by deregulating homeostasis.
The effects of Porphyromonas gingivalis infection before and at different gestation periods were evaluated.
Wistar rats infected via subcutaneous with P. gingivalis W83, one week before mating (BM), days 1 (PR1) and 11 of gestation (PR11), and controls were evaluated, and samples were obtained at the end of gestation. P. gingivalis was detected by PCR. Cytokine was determined by ELISA.
Infected rats had lower maternal gain of weight. Implantation was not observed in 2/12 BM rats. PR11 presented more fetal-placental resorptions and lower placenta/fetus weight than controls. P. gingivalis was detected in placenta and fetus. IL-6 and TNF-α levels were higher in placenta and serum of infected groups, except for TNF-α in placenta of PR1. IL-1β levels were higher in placenta of PR11, but lower in serum and placenta of PR1. There were no differences in IL-10 and PGE2 concentrations among the groups (P < 0.05).
The experimental infection by P. gingivalis resulted in alterations in the gestational pattern and in fetal development. The consequences of infection at mid-gestation were more severe than at the beginning, possibly due to the induction of pro-inflammatory cytokines in the fetal compartment.
感染因子可能通过破坏内稳状态而影响妊娠结局。
评估牙龈卟啉单胞菌在妊娠前和不同妊娠时期感染的影响。
Wistar 大鼠通过皮下接种牙龈卟啉单胞菌 W83 进行感染，在交配前一周（BM）、妊娠第 1 天（PR1）和第 11 天（PR11）进行感染，设立对照组，并在妊娠末期采集样本。通过 PCR 检测牙龈卟啉单胞菌。通过 ELISA 测定细胞因子。
感染大鼠的母体体重增加减少。BM 组的 2/12 只大鼠未观察到胚胎着床。PR11 组的胎儿胎盘吸收和胎盘/胎儿重量较对照组降低。在胎盘和胎儿中检测到牙龈卟啉单胞菌。感染组胎盘和血清中的 IL-6 和 TNF-α水平升高，但 PR1 组胎盘中的 TNF-α除外。PR11 组胎盘中的 IL-1β水平升高，但血清和胎盘中的 IL-1β水平降低。各组间 IL-10 和 PGE2 浓度无差异（P < 0.05）。
牙龈卟啉单胞菌的实验性感染导致妊娠模式和胎儿发育的改变。妊娠中期感染的后果比妊娠早期更严重，这可能是由于胎儿部位促炎细胞因子的诱导。
