Boggess Kim A, Madianos Phoebus N, Preisser John S, Moise Kenneth J, Offenbacher Steven
Department of Obstetrics and Gynecology, University of North Carolina School of Medicine, Chapel Hill, NC, USA.
Am J Obstet Gynecol. 2005 Feb;192(2):554-7. doi: 10.1016/j.ajog.2004.09.001.
This study was undertaken to develop a rabbit model of maternal exposure to Porphyromonas gingivalis and determine whether fetal or placental exposure occurs.
Subcutaneous steel chambers were implanted in 8 New Zealand White female rabbits. On day 7 of pregnancy, 4 rabbits were inoculated through the chamber with 5 x 10 8 CFU/mL live P gingivalis , and 4 rabbits with broth (controls) and sacrificed at term. Polymerase chain reaction was used to detect P gingivalis in maternal and fetal liver and placenta. Fisher exact test was used to compare P gingivalis detection between groups.
Among exposed does, P gingivalis was detected in 33% of the maternal livers, 49% of placentas, and 34% fetal livers compared with none from controls ( P < .001).
Chronic maternal exposure to P gingivalis results in systemic dissemination, transplacental passage, and fetal exposure. This model may be useful to study placental and fetal effects of this oral pathogen and to study microbial dissemination across the placenta.
本研究旨在建立母兔暴露于牙龈卟啉单胞菌的模型，并确定胎儿或胎盘是否会受到暴露。
在8只新西兰白兔母兔体内植入皮下钢室。在怀孕第7天，4只母兔通过钢室接种5×10⁸CFU/mL的牙龈卟啉单胞菌活菌，4只母兔接种肉汤（对照组），足月时处死。采用聚合酶链反应检测母兔、胎儿肝脏和胎盘内的牙龈卟啉单胞菌。采用Fisher精确检验比较两组间牙龈卟啉单胞菌的检测情况。
在暴露组母兔中，33%的母兔肝脏、49%的胎盘和34%的胎儿肝脏检测到牙龈卟啉单胞菌，而对照组均未检测到（P<0.001）。
母兔长期暴露于牙龈卟啉单胞菌会导致全身播散、经胎盘传播和胎儿暴露。该模型可能有助于研究这种口腔病原体对胎盘和胎儿的影响，以及研究微生物在胎盘内的传播。
