Bélanger Myriam, Reyes Leticia, von Deneen Karen, Reinhard Mary K, Progulske-Fox Ann, Brown Mary B
Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32611-0880, USA.
Am J Obstet Gynecol. 2008 Jul;199(1):86.e1-7. doi: 10.1016/j.ajog.2007.11.067. Epub 2008 Mar 20.
The objective of this study was to develop a rodent model of Porphyromonas gingivalis infection during pregnancy.
Sprague Dawley rats were infected intravenously with 10(5), 10(7), or 10(9) CFU per dam of P gingivalis strain W83, ATCC 33277, or A7436 at gestational day 14 and necropsied at gestational day 18. Maternal organs were cultured to assess the spread of the infection. Six fetal units (placenta, amniotic fluid, membranes, and fetus) per dam were cultured; additional fetal units were examined by histopathology. Polymerase chain reaction was performed on placentas.
Colonization rates were dependent on the strain of P gingivalis used and the infection dose. At an infection dose of 10(9) CFU/dam, P gingivalis W83, ATCC 33277, or A7436 was detected in 33%, 83%, or 100% of placentas, respectively. Epithelial hyperplasia, cellular necrosis, and inflammatory infiltrate were observed in infected placental tissues.
This study demonstrated that P gingivalis can invade both maternal and fetal tissues, resulting in chorioamnionitis and placentitis.
本研究的目的是建立孕期牙龈卟啉单胞菌感染的啮齿动物模型。
在妊娠第14天，将每只孕鼠10⁵、10⁷或10⁹CFU的牙龈卟啉单胞菌W83菌株、ATCC 33277菌株或A7436菌株经静脉感染Sprague Dawley大鼠，并在妊娠第18天进行尸检。对母体器官进行培养以评估感染的扩散情况。对每只孕鼠的6个胎儿单位（胎盘、羊水、胎膜和胎儿）进行培养；对额外的胎儿单位进行组织病理学检查。对胎盘进行聚合酶链反应。
定植率取决于所用牙龈卟啉单胞菌的菌株和感染剂量。在感染剂量为10⁹CFU/孕鼠时，牙龈卟啉单胞菌W83菌株、ATCC 33277菌株或A7436菌株分别在33%、83%或100%的胎盘中被检测到。在感染的胎盘组织中观察到上皮增生、细胞坏死和炎性浸润。
本研究表明牙龈卟啉单胞菌可侵入母体和胎儿组织，导致绒毛膜羊膜炎和胎盘炎。
