Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104-4539, USA.
Cell Transplant. 2012;21(10):2189-200. doi: 10.3727/096368912X636821. Epub 2012 Apr 2.
Clinical trials utilizing bone marrow-derived mesenchymal stem cell (BM-MSC) therapies show promise for treating a variety of pathologic conditions. Paramount to optimization of such cell-based therapies is a thorough understanding of MSC biology. Despite the tremendous potential that exists for the clinical use of canine BM-MSCs in veterinary medicine, as well as in preclinical studies for human medicine, relatively little information exists regarding basic biological properties of the cells. In this study, we compared the importance of donor characteristics (age and harvest site) and ex vivo expansion on canine BM-MSC frequency (CFU-f) and differentiation potential. Advancing age was found to have a negative effect on CFU-f as well as osteogenic potential. Site of harvest was also found to have significant effects on MSC properties. MSCs obtained from the humerus were found at the lowest frequency and were least osteogenic compared to those harvested from the tibia, femur, and ilium. Osteogenic potential diminished significantly by the third passage. These results suggest important donor parameters and culture effects to consider in translational studies examining MSC-based regenerative medical strategies.
临床研究利用骨髓间充质干细胞（BM-MSC）疗法显示出治疗多种病理状况的潜力。优化此类基于细胞的疗法的关键是彻底了解 MSC 生物学。尽管犬骨髓间充质干细胞在兽医医学以及人类医学的临床前研究中具有巨大的应用潜力，但关于细胞的基本生物学特性的信息相对较少。在这项研究中，我们比较了供体特征（年龄和采集部位）和体外扩增对犬骨髓间充质干细胞集落形成单位（CFU-f）和分化潜能的重要性。发现年龄的增长对 CFU-f 和成骨潜能有负面影响。采集部位也对 MSC 特性有显著影响。与从胫骨、股骨和髂骨采集的 MSC 相比，从肱骨采集的 MSC 频率最低，成骨潜能最低。成骨潜能在第三代时显著降低。这些结果表明，在检查基于 MSC 的再生医学策略的转化研究中，需要考虑重要的供体参数和培养影响。
