Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, RS, Brasil.
Braz J Med Biol Res. 2012 May;45(5):436-43. doi: 10.1590/s0100-879x2012007500052. Epub 2012 Apr 5.
The objective of this study was to identify the possible involvement of the GABAergic system in the anesthetic effect of Lippia alba essential oil (EO). We propose a new animal model using silver catfish (Rhamdia quelen) exposed to an anesthetic bath to study the mechanism of action of EO. To observe the induction and potentiation of the anesthetic effect of EO, juvenile silver catfish (9.30 ± 1.85 g; 10.15 ± 0.95 cm; N = 6) were exposed to various concentrations of L. alba EO in the presence or absence of diazepam [an agonist of high-affinity binding sites for benzodiazepinic (BDZ) sites coupled to the GABA A receptor complex]. In another experiment, fish (N = 6) were initially anesthetized with the EO and then transferred to an anesthetic-free aquarium containing flumazenil (a selective antagonist of binding sites for BDZ coupled to the GABA A receptor complex) or water to assess recovery time from the anesthesia. In this case, flumazenil was used to observe the involvement of the GABA-BDZ receptor in the EO mechanism of action. The results showed that diazepam potentiates the anesthetic effect of EO at all concentrations tested. Fish exposed to diazepam and EO showed faster recovery from anesthesia when flumazenil was added to the recovery bath (12.0 ± 0.3 and 7.2 ± 0.7, respectively) than those exposed to water (9.2 ± 0.2 and 3.5 ± 0.3, respectively). In conclusion, the results demonstrated the involvement of the GABAergic system in the anesthetic effect of L. alba EO on silver catfish.
本研究旨在确定 GABA 能系统是否参与了 Lippia alba 精油（EO）的麻醉作用。我们提出了一个新的动物模型，使用银鲶（Rhamdia quelen）暴露于麻醉浴中，以研究 EO 的作用机制。为了观察 EO 麻醉作用的诱导和增强，幼银鲶（9.30 ± 1.85 g；10.15 ± 0.95 cm；N = 6）在存在或不存在地西泮（一种与 GABA A 受体复合物偶联的高亲和力结合位点的苯二氮䓬（BDZ）配体激动剂）的情况下，暴露于不同浓度的 L. alba EO 中。在另一个实验中，鱼（N = 6）最初用 EO 麻醉，然后转移到不含麻醉剂的水族馆中，该水族馆含有氟马西尼（一种与 GABA A 受体复合物偶联的 BDZ 结合位点的选择性拮抗剂）或水，以评估从麻醉中恢复的时间。在这种情况下，氟马西尼用于观察 GABA-BDZ 受体在 EO 作用机制中的参与。结果表明，地西泮在所有测试浓度下均增强了 EO 的麻醉作用。当在恢复浴中添加氟马西尼时，暴露于地西泮和 EO 的鱼从麻醉中恢复的速度更快（分别为 12.0 ± 0.3 和 7.2 ± 0.7），而暴露于水的鱼恢复速度较慢（分别为 9.2 ± 0.2 和 3.5 ± 0.3）。总之，结果表明 GABA 能系统参与了 L. alba EO 对银鲶的麻醉作用。
