Sollod A E, Frank G H
Am J Vet Res. 1979 May;40(5):658-64.
Yearling cattle were inoculated with a recently isolated field strain of Trypanosoma congolense. Dinitrophenylated ovalbumin, a bacteriophage, or bovine parainfluenza-3 virus injected into the cattle during the first 5 weeks of infection resulted in peak serum antibody titers lower but not much lower than those produced by noninfected cattle. Primary and secondary antibody responses of inoculated cattle required more time to reach peak titers. Peripheral blood lymphocyte concentrations decreased to 42% of preinfection base-line value 1 1/2 weeks after the onset of T congolense infection and thereafter plateaued at less than 75% of base-line value. Lymphocyte cultures prepared from infected and noninfected cattle gave no marked differences in [(3)H]thymidine uptake after stimulation with phytohemagglutinin or pokeweed mitogen. Differences in lymphpocyte responsiveness were not noticed in one-way mixed lymphocyte culture reactions, using mitomycin C-treated lymphocytes from noninfected cattle as stimulator cells.
一岁的牛接种了最近分离出的刚果锥虫野外菌株。在感染的前5周内，将二硝基苯基化卵清蛋白、一种噬菌体或牛副流感3病毒注射到牛体内，导致血清抗体滴度峰值低于未感染牛产生的峰值，但降低幅度不大。接种牛的初次和二次抗体反应需要更多时间才能达到峰值滴度。刚果锥虫感染开始1.5周后，外周血淋巴细胞浓度降至感染前基线值的42%，此后稳定在基线值的75%以下。用植物血凝素或商陆丝裂原刺激后，从感染和未感染牛制备的淋巴细胞培养物在[³H]胸腺嘧啶核苷摄取方面没有显著差异。在单向混合淋巴细胞培养反应中，未观察到淋巴细胞反应性的差异，该反应使用来自未感染牛的经丝裂霉素C处理的淋巴细胞作为刺激细胞。
