感染刚果锥虫的牛白细胞对丝裂原的体外反应。
The in vitro response to mitogens of leucocytes from cattle infected with Trypanosoma congolense.
作者信息
Masake R A, Pearson T W, Wells P, Roelants G E
出版信息
Clin Exp Immunol. 1981 Mar;43(3):583-9.
The in vitro proliferative response of bovine leucocytes from peripheral blood to LPS, PWM and Con A was monitored during the course of infection with T. congolense. The stimulatory effect of LPS and PWM on PBL was significantly decreased in infected cattle while the reduction of stimulatory response to Con A was less marked. There was a high background proliferation of unstimulated leucocytes from infected cattle. This increase in background counts correlated with the parasitaemia in that both features were abolished by treatment with the trypanocidal drug Berenil (4,4'-diamidino-diazoaminobenzene-diacetamide acetone). Co-cultivation of trypanosomes with PBL from normal cattle did not depress the responses of the latter to mitogens, and trypanosomes maintained in a cell-free medium failed to incorporate 14C-thymidine under the conditions used.
在感染刚果锥虫的过程中，监测了来自外周血的牛白细胞对脂多糖（LPS）、美洲商陆有丝分裂原（PWM）和刀豆蛋白A（Con A）的体外增殖反应。在受感染的牛中，LPS和PWM对外周血淋巴细胞（PBL）的刺激作用显著降低，而对Con A刺激反应的降低则不太明显。受感染牛的未刺激白细胞有较高的背景增殖。背景计数的这种增加与寄生虫血症相关，因为这两个特征在用杀锥虫药物贝尼尔（4,4'-二脒基-重氮氨基苯-二乙酰胺丙酮）治疗后均消失。将锥虫与正常牛的PBL共同培养不会抑制后者对有丝分裂原的反应，并且在所用条件下，保存在无细胞培养基中的锥虫不能掺入14C-胸腺嘧啶核苷。
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