Center for Biomedical EPR Spectroscopy and Imaging, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.
Magn Reson Med. 2013 Feb;69(2):594-601. doi: 10.1002/mrm.24250. Epub 2012 Apr 3.
In vivo or ex vivo electron paramagnetic resonance imaging (EPRI) is a powerful technique for determining the spatial distribution of free radicals and other paramagnetic species in living organs and tissues. However, applications of EPRI have been limited by long projection acquisition times and the consequent fact that rapid gated EPRI was not possible. Hence in vivo EPRI typically provided only time-averaged information. In order to achieve direct gated EPRI, a fast EPR acquisition scheme was developed to decrease EPR projection acquisition time down to 10-20 ms, along with corresponding software and instrumentation to achieve fast gated EPRI of the isolated beating heart with submillimeter spatial resolution in as little as 2-3 min. Reconstructed images display temporal and spatial variations of the free-radical distribution, anatomical structure, and contractile function within the rat heart during the cardiac cycle.
在体或离体电子顺磁共振成像（EPRI）是一种强大的技术，可用于确定活器官和组织中自由基和其他顺磁物种的空间分布。然而，EPRI 的应用受到长投影采集时间的限制，因此不可能进行快速门控 EPRI。因此，在体 EPRI 通常仅提供时间平均信息。为了实现直接门控 EPRI，开发了一种快速 EPR 采集方案，将 EPR 投影采集时间缩短至 10-20 毫秒，同时还开发了相应的软件和仪器设备，以便在短短 2-3 分钟内以亚毫米的空间分辨率对分离的跳动心脏进行快速门控 EPRI。重建图像显示了在心动周期中大鼠心脏内自由基分布、解剖结构和收缩功能的时间和空间变化。
