Laboratory of Neuroendocrinology, The Rockefeller University, New York, NY 10065, USA.
Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6175-80. doi: 10.1073/pnas.1203941109. Epub 2012 Apr 2.
Dendritic cells (DC) are antigen-presenting cells found in both lymphoid and nonlymphoid organs, including the brain (bDC) of Cd11c/eyfp transgenic C57BL/6 mice. Using an intranasal vesicular stomatitis virus infection, we demonstrated that EYFP(+) cells amass in areas associated with viral antigens, take on an activated morphology, and project their processes into infected neuronal tissue within the olfactory bulb. These bDC separated into three EYFP(+) CD45(+) CD11b(+) populations, all but one being able to functionally promote both T lymphocyte proliferation and T(H)1 cytokine production. One population was shown to emanate from the brain and a second population was peripherally derived. The third population was of indeterminate origin, being both radiosensitive and not replenished by donor bone marrow. Finally, each EYFP(+) population contained CD11b(+) CD103(+) subpopulations and could be distinguished in terms of CD115, Gr-1, and Ly-6C expression, highlighting mucosal and monocyte-derived DC lineages.
树突状细胞（DC）是一种抗原呈递细胞，存在于淋巴器官和非淋巴器官中，包括 Cd11c/eyfp 转基因 C57BL/6 小鼠的大脑（bDC）。我们使用鼻内水疱性口炎病毒感染证明，EYFP(+)细胞聚集在与病毒抗原相关的区域，呈现出激活的形态，并将其突起延伸到嗅球内感染的神经元组织中。这些 bDC 分为三个 EYFP(+) CD45(+) CD11b(+)群体，除了一个群体外，其他群体都能够有效地促进 T 淋巴细胞增殖和 T(H)1 细胞因子的产生。一个群体被证明来源于大脑，第二个群体来源于外周。第三个群体的来源不确定，既对辐射敏感，也不能被供体骨髓补充。最后，每个 EYFP(+)群体都包含 CD11b(+) CD103(+)亚群，并且可以根据 CD115、Gr-1 和 Ly-6C 的表达来区分，突出了黏膜和单核细胞衍生的 DC 谱系。
