利用两个叶绿体基因 rbcL 和 matK 对石斛属乔伊氏兜兰（兰科）的亲子关系进行鉴定。

Parentage determination of Vanda Miss Joaquim (Orchidaceae) through two chloroplast genes rbcL and matK.

机构信息

Singapore Botanic Gardens, National Parks Board, 1 Cluny Road, Singapore 259569 , Singapore.

出版信息

AoB Plants. 2011;2011:plr018. doi: 10.1093/aobpla/plr018. Epub 2011 Aug 16.

DOI:10.1093/aobpla/plr018

PMID:22476488

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3156982/

Abstract

BACKGROUND AND AIMS

The popular hybrid orchid Vanda Miss Joaquim was made Singapore's national flower in 1981. It was originally described in the Gardeners' Chronicle in 1893, as a cross between Vanda hookeriana and Vanda teres. However, no record had been kept as to which parent contributed the pollen. This study was conducted using DNA barcoding techniques to determine the pod parent of V. Miss Joaquim, thereby inferring the pollen parent of the hybrid by exclusion.

METHODOLOGY

Two chloroplast genes, matK and rbcL, from five related taxa, V. hookeriana, V. teres var. alba, V. teres var. andersonii, V. teres var. aurorea and V. Miss Joaquim 'Agnes', were sequenced. The matK gene from herbarium specimens of V. teres and V. Miss Joaquim, both collected in 1893, was also sequenced.

PRINCIPAL RESULTS

No sequence variation was found in the 600-bp region of rbcL sequenced. Sequence variation was found in the matK gene of V. hookeriana, V. teres var. alba, V. teres var. aurorea and V. Miss Joaquim 'Agnes'. Complete sequence identity was established between V. teres var. andersonii and V. Miss Joaquim 'Agnes'. The matK sequences obtained from the herbarium specimens of V. teres and V. Miss Joaquim were completely identical to the sequences obtained from the fresh samples of V. teres var. andersonii and V. Miss Joaquim 'Agnes'.

CONCLUSIONS

The pod parent of V. Miss Joaquim 'Agnes' is V. teres var. andersonii and, by exclusion, the pollen parent is V. hookeriana. The herbarium and fresh samples of V. teres var. andersonii and V. Miss Joaquim share the same inferred maternity. The matK gene was more informative than rbcL and facilitated differentiation of varieties of V. teres.

摘要

背景和目的

受欢迎的杂交兰花 Vanda Miss Joaquim 于 1981 年被选为新加坡国花。它最初于 1893 年在《园丁纪事》中被描述为 Vanda hookeriana 和 Vanda teres 的杂交种。然而，没有记录表明哪个亲本贡献了花粉。本研究使用 DNA 条形码技术来确定 V. Miss Joaquim 的母本，从而通过排除法推断杂交种的父本花粉。

方法

从五个相关种，V. hookeriana、V. teres var. alba、V. teres var. andersonii、V. teres var. aurorea 和 V. Miss Joaquim 'Agnes' 中测序了两个叶绿体基因 matK 和 rbcL。还对 1893 年采集的 V. teres 和 V. Miss Joaquim 的标本中的 matK 基因进行了测序。

主要结果

rbcL 测序的 600bp 区域未发现序列变异。V. hookeriana、V. teres var. alba、V. teres var. aurorea 和 V. Miss Joaquim 'Agnes' 的 matK 基因中发现了序列变异。V. teres var. andersonii 和 V. Miss Joaquim 'Agnes' 之间建立了完全的序列同一性。从 V. teres 和 V. Miss Joaquim 的标本中获得的 matK 序列与从 V. teres var. andersonii 和 V. Miss Joaquim 'Agnes' 的新鲜样本中获得的序列完全相同。

结论

V. Miss Joaquim 'Agnes' 的母本是 V. teres var. andersonii，通过排除法，父本是 V. hookeriana。V. teres var. andersonii 和 V. Miss Joaquim 的标本和新鲜样本具有相同的母系亲缘关系。matK 基因比 rbcL 更具信息量，有助于区分 V. teres 的品种。