Faculty of Medicine Carl Gustav Carus, Dresden University of Technology, Clinical Sensoring and Monitoring, 01307, Dresden, Germany.
Anal Bioanal Chem. 2012 May;403(3):727-35. doi: 10.1007/s00216-012-5824-y. Epub 2012 Apr 5.
Fourier transform infrared (FT-IR) spectroscopic imaging has been used to characterize different types of pituitary gland tumors and normal pituitary tissue. Freshly resected tumor tissue from surgery was prepared as thin cryosections and examined by FT-IR spectroscopic imaging. Tissue types were discriminated via k-means cluster analysis and a supervised classification algorithm based on linear discriminant analysis. Spectral classification allowed us to discriminate between tumor and non-tumor cells, as well as between tumor cells that produce human growth hormone (hGH+) and tumor cells that do not produce that hormone (hGH-). The spectral classification was compared and contrasted with a histological PAS and orange G stained image. It was further shown that hGH+ pituitary tumor cells show stronger amide bands than tumor cells that do not produce hGH. This study demonstrates that FT-IR spectroscopic imaging can not only potentially serve as a fast and objective approach for discriminating pituitary gland tumors from normal tissue, but that it can also detect hGH-producing tumor cells.
傅里叶变换红外（FT-IR）光谱成像已被用于表征不同类型的垂体瘤和正常垂体组织。从手术中切除的新鲜肿瘤组织被制备成薄的冷冻切片，并通过 FT-IR 光谱成像进行检查。通过 k-均值聚类分析和基于线性判别分析的有监督分类算法对组织类型进行区分。光谱分类允许我们区分肿瘤细胞和非肿瘤细胞，以及产生人生长激素（hGH+）的肿瘤细胞和不产生该激素的肿瘤细胞（hGH-）。将光谱分类与组织学 PAS 和橙色 G 染色图像进行了比较和对比。进一步表明，hGH+垂体瘤细胞的酰胺带比不产生 hGH 的肿瘤细胞更强。这项研究表明，FT-IR 光谱成像不仅可以作为一种快速、客观的方法来区分垂体瘤和正常组织，而且可以检测产生 hGH 的肿瘤细胞。
