Food Engineering & Technology Department, Institute of Chemical Technology, Nathalal Parikh Marg, Matunga, Mumbai 400019, India.
Appl Biochem Biotechnol. 2012 Jul;167(6):1811-7. doi: 10.1007/s12010-012-9658-z. Epub 2012 Apr 4.
Dioscorea alata var. purpurae (Indian purple yam) is an important source of diosgenin, a triterpenoid that is used as a raw material in the synthesis of corticosteroid hormones. These drugs are used for the treatment of pharmacological conditions such as arthritis. This paper reports in vitro propagation of explants of various parts of Dioscorea-tuber, leaves, and nodes. Murashige and Skoog media supplemented with hormones and additives was used to get maximum callus initiation and shoot/root induction. All the cultures were maintained at 25 ± 2 °C under cool-white fluorescent tubes with 16-h photoperiod. Callus initiation was observed from 8th to 11th day of inoculation, and subsequent root/shoot was initiated in nodal callus after 21 days. Hormones such as kinetin, indole-3-acetic acid, indole-3-butyric acid, α-naphthalene acetic acid, and thiadizuron did not show significant enhancement. Also, there was no need for supplementing additives (silver nitrate, glutamine, L-: asparagine monohydrate, polyethylene glycol). Combination of 6-benzylaminopurine (0.2 ppm) and 2,4-dichlorophenoxyacetic acid (2 ppm) hormones gave the best results, and all parts of the plants gave similar callus induction.
紫萁（Dioscorea alata var. purpurae）是薯蓣皂素的重要来源，薯蓣皂素是合成皮质甾类激素的原料。这些药物用于治疗关节炎等药理学病症。本文报道了紫萁各个部位（块茎、叶片和节）外植体的离体繁殖。使用添加有激素和添加剂的 Murashige 和 Skoog 培养基，以获得最大的愈伤组织起始和芽/根诱导。所有培养物均在 25°C ± 2°C、冷白色荧光灯下，以 16 小时的光周期保持。接种后第 8-11 天观察到愈伤组织起始，21 天后节位愈伤组织中开始生根/发芽。细胞激动素、吲哚-3-乙酸、吲哚-3-丁酸、α-萘乙酸和噻二唑隆等激素没有明显的增强作用。此外，也不需要添加添加剂（硝酸银、谷氨酰胺、L-：天门冬酰胺一水合物、聚乙二醇）。6-苄基腺嘌呤（0.2ppm）和 2,4-二氯苯氧乙酸（2ppm）激素的组合效果最佳，且植物的各个部位都能产生相似的愈伤组织诱导。
