University of Maryland, College Park, MD 20742, USA.
Trends Biotechnol. 2012 May;30(5):291-300. doi: 10.1016/j.tibtech.2012.02.003. Epub 2012 Apr 3.
Electrospray-differential mobility analysis (ES-DMA) is a versatile technique used to aerosolize bionanoparticles and measure their electrical mobility at ambient conditions. ES-DMA is similar to electrospray-mass spectrometry (ES-MS), but measures the effective particle size, rather than mass. It has a wide range of applications and nominally can be used to characterize biomolecules and nanoparticles ranging in size from a few nanometers (~3 nm) to several hundred nanometers, to obtain multimodal size distributions in minutes. Although both the ES and the DMA are mature technologies, they are finding increased use in combination to characterize particles in liquids. In this paper, we review ES-DMA, and how it has recently been used to characterize bionanoparticles such as polymers, proteins, viruses, bacteriophages and nanoparticle-biomolecule conjugates.
电喷雾-差分迁移率分析 (ES-DMA) 是一种通用技术，用于将生物纳米颗粒气溶胶化，并在环境条件下测量它们的电迁移率。ES-DMA 类似于电喷雾质谱 (ES-MS)，但测量的是有效粒径，而不是质量。它有广泛的应用，名义上可以用于表征大小从几纳米 (~3nm) 到几百纳米的生物分子和纳米颗粒，在几分钟内获得多模态的尺寸分布。尽管 ES 和 DMA 都是成熟的技术，但它们在组合使用方面的应用越来越多，用于表征液体中的颗粒。本文综述了 ES-DMA，以及它最近如何用于表征聚合物、蛋白质、病毒、噬菌体和纳米颗粒-生物分子缀合物等生物纳米颗粒。
