Dept. of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Langenbeckstrasse 1, Mainz,Germany.
J Proteomics. 2012 Jun 18;75(11):3177-90. doi: 10.1016/j.jprot.2012.03.018. Epub 2012 Mar 24.
Tear proteomic studies revealed distinct similarities between contact lens wearers and dry eye patients. AMO Complete® multipurpose contact lens cleaning solutions containing taurine seem to have a beneficial effect regarding contact lens induced dry eye. To illuminate the effect of taurine on the tear proteome of contact lens wearers and sicca patients we developed a gel-based RP-RP capillary HPLC-MALDI TOF/TOF MS strategy. Two contact lens wearer groups, one using eye drops containing 0.05% taurine; the other for control physiological NaCl solution were monitored. Also, a third group of sicca patients using taurine solution was studied (N=4 individuals/group). Tear pools of each group at six time points over 5 weeks were analyzed. In summary 267 tear proteins were identified. We found a protein subset showing a linear taurine response with R(2) values ≥0.5. Taurine effects were detected predominantly in the contact lens group demonstrated by distinct level decreases. Most protein candidates were related to inflammation. Since levels of these proteins differentiate from those of a healthy non-contact lens wearer reference they are supposed to be involved in contact lens induced dry eye and should be focused on in further studies.
泪液蛋白质组学研究表明，隐形眼镜佩戴者和干眼症患者之间存在明显的相似之处。含有牛磺酸的 AMO Complete®多功能隐形眼镜清洁溶液似乎对隐形眼镜引起的干眼症有有益的影响。为了阐明牛磺酸对隐形眼镜佩戴者和干燥症患者泪液蛋白质组的影响，我们开发了一种基于凝胶的 RP-RP 毛细管 HPLC-MALDI TOF/TOF MS 策略。监测了两组隐形眼镜佩戴者，一组使用含有 0.05%牛磺酸的滴眼液；另一组作为对照使用生理氯化钠溶液。此外，还研究了第三组使用牛磺酸溶液的干燥症患者（每组 N=4 人）。在 5 周的 6 个时间点分析了每组的泪液池。总之，鉴定出了 267 种眼泪蛋白。我们发现了一组具有线性牛磺酸反应的蛋白质亚群，其 R(2)值≥0.5。通过明显的水平降低，发现了主要在隐形眼镜组中检测到的牛磺酸作用。大多数蛋白质候选物与炎症有关。由于这些蛋白质的水平与健康非隐形眼镜佩戴者的参考值不同，因此它们可能与隐形眼镜引起的干眼症有关，应该在进一步的研究中加以关注。
