Tao Wen, Soonpaa Mark H, Field Loren J, Chen Peng-Sheng, Firulli Anthony B, Shou Weinian, Rubart Michael
Department of Pediatrics, Riley Heart Research Center, Indiana University School of Medicine, 1044 West Walnut Street, Indianapolis, IN 46202, USA.
Pediatr Cardiol. 2012 Aug;33(6):929-37. doi: 10.1007/s00246-012-0314-8. Epub 2012 Apr 6.
Although the adult mammalian myocardium exhibits a limited ability to undergo regenerative growth, its intrinsic renewal rate is insufficient to compensate for myocyte loss during cardiac disease. Transplantation of donor cardiomyocytes or cardiomyogenic stem cells is considered a promising strategy for reconstitution of cardiac mass, provided the engrafted cells functionally integrate with host myocardium and actively contribute to its contractile force. The authors previously developed a two-photon fluorescence microscopy-based assay that allows in situ screening of donor cell function after intracardiac delivery of the cells. This report reviews the techniques of two-photon fluorescence microscopy and summarizes its application for quantifying the extent to which a variety of donor cell types stably and functionally couple with the recipient myocardium.
尽管成年哺乳动物心肌的再生生长能力有限，但其内在更新率不足以弥补心脏疾病期间的心肌细胞损失。供体心肌细胞或心肌生成干细胞的移植被认为是重建心脏质量的一种有前景的策略，前提是植入的细胞能与宿主心肌在功能上整合并积极贡献于其收缩力。作者之前开发了一种基于双光子荧光显微镜的检测方法，可在心脏内递送细胞后对供体细胞功能进行原位筛选。本报告回顾了双光子荧光显微镜技术，并总结了其在量化多种供体细胞类型与受体心肌稳定和功能耦合程度方面的应用。
