Institute for Medical Microbiology and Hygiene, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.
Mediators Inflamm. 2012;2012:849136. doi: 10.1155/2012/849136. Epub 2012 Jan 26.
The formation of neutrophil extracellular traps (NETs) depends on the generation of reactive oxygen species (ROS). Previous studies revealed that both NADPH oxidase and myeloperoxidase (MPO) are required for NET release. However, the contribution of various ROS as well as the role of mitochondria-derived ROS has not been addressed so far. In the present study we aimed to investigate in a systematic and comprehensive manner the contribution of various ROS and ROS-generating pathways to the PMA-induced NET release. By using specific inhibitors, the role of both NADPH oxidase- and mitochondria-derived ROS as well as the contribution of superoxide dismutase (SOD) and MPO on the NET release was assessed. We could demonstrate that NADPH oxidase function is crucial for the formation of NETs. In addition, we could clearly show the involvement of MPO-derived ROS in NET release. Our results, however, did not provide evidence for the role of SOD- or mitochondria-derived ROS in NET formation.
中性粒细胞胞外诱捕网（NETs）的形成依赖于活性氧（ROS）的产生。先前的研究表明，NETs 的释放既需要 NADPH 氧化酶又需要髓过氧化物酶（MPO）。然而，到目前为止，各种 ROS 的贡献以及线粒体来源的 ROS 的作用尚未得到解决。在本研究中，我们旨在系统全面地研究各种 ROS 和 ROS 生成途径对 PMA 诱导的 NET 释放的贡献。通过使用特定的抑制剂，评估了 NADPH 氧化酶和线粒体来源的 ROS 的作用以及超氧化物歧化酶（SOD）和 MPO 的贡献对 NET 释放的影响。我们可以证明 NADPH 氧化酶的功能对于 NET 的形成至关重要。此外，我们可以清楚地表明 MPO 衍生的 ROS 参与了 NET 的释放。然而，我们的结果并没有提供 SOD 或线粒体来源的 ROS 在 NET 形成中作用的证据。
