Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Departamento de Fitotecnia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Genet Mol Biol. 2012 Jan;35(1):172-81. doi: 10.1590/s1415-47572012005000005. Epub 2012 Jan 20.
Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil. Thus eight embryogenesis-regulating genes, viz., ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semi-quantitative RT-PCR during embryo development and germination. All the eight were found to be differentially expressed in the various developmental stages of zygotic embryos, seeds and seedling tissues. To our knowledge, this is the first report on embryogenesis-regulating gene expression in members of the Araucariaceae family, as well as in plants with recalcitrant seeds.
被子植物和裸子植物大约在 3 亿年前从一个共同的祖先进化而来。除了胚胎发生和种子起源的形态和结构差异外，在胚胎发育中涉及的一组胚胎发生调节基因和分子机制似乎在两个类群中都得到了很好的保守。很少有研究涵盖巴西松树（巴西唯一具有经济重要性的本地针叶树）胚胎发生的分子方面。因此，通过半定量 RT-PCR 分析了 8 个胚胎发生调节基因，即 ARGONAUTE 1、CUP-SHAPED COTYLEDON 1、WUSCHEL 相关 WOX、S-LOCUS LECTIN PROTEIN KINASE、SCARECROW-like、VICILIN 7S、LEAFY COTYLEDON 1 和 REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1，在胚胎发育和萌发过程中的各个发育阶段。所有这 8 个基因在合子胚、种子和幼苗组织的不同发育阶段都表现出差异表达。据我们所知，这是首次报道 Araucariaceae 科成员以及种子休眠植物的胚胎发生调节基因表达。
